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Invitrogen
PDX1 Polyclonal Antibody
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FIGURE: 1 / 1
PDX1 Antibody (PA5-87695) in WB
Western blot analysis of PDX1 in extracts of various cell lines. Samples were incubated with PDX1 Polyclonal antibody (Product # PA5-87695) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87695
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PDX1 (NP_0002001)
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Amount
62 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MNGEEQYYAA TQLYKDPCAF QRGPAPEFSA SPPACLYMGR QPPPPPPHPF PGALGALEQG SPPDISPYEV PPLADDPAVA HLHHHLPAQL ALPHPPAGPF; Positive Samples: DU145, HepG2, Mouse pancreas, Mouse liver; Cellular Location: Cytoplasm, Nucleus, cytosol
Target Information
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DLDBP; E3BP; Glucose-sensitive factor; GSF; IDX-1; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF-1; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF-1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox gene 1; pancreatic-duodenal homeobox factor 1; PDHX; PDX-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1
Gene Aliases: GSF; IDX-1; IPF-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; PDX1; STF-1; STF1
UniProt ID: (Human) P52945, (Mouse) P52946
Entrez Gene ID: (Human) 3651, (Mouse) 18609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
macromolecular complex binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
transcription factor binding
protein complex binding
protein heterodimerization activity
negative regulation of transcription from RNA polymerase II promoter
liver development
type B pancreatic cell differentiation
glucose metabolic process
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
smoothened signaling pathway
nitric oxide mediated signal transduction
multicellular organism development
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
regulation of gene expression
positive regulation of cell death
morphogenesis of embryonic epithelium
insulin secretion
cell differentiation
pancreas development
exocrine pancreas development
endocrine pancreas development
positive regulation of insulin secretion
positive regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of cell proliferation
glucose homeostasis
positive regulation of DNA binding
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
stem cell differentiation
detection of glucose
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
negative regulation of type B pancreatic cell apoptotic process
generation of precursor metabolites and energy
cell proliferation
response to xenobiotic stimulus
organ morphogenesis
response to iron(II) ion
response to chlorate
glucose mediated signaling pathway
organ regeneration
response to nutrient levels
response to vitamin
response to lipid
response to cytokine
response to nicotine
enteroendocrine cell differentiation
response to leucine
response to alkaloid
type B pancreatic cell proliferation
epithelial cell proliferation
negative regulation of epithelial cell proliferation
response to glucocorticoid
transdifferentiation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
response to fatty acid
type B pancreatic cell apoptotic process
cellular response to acadesine
positive regulation of type B pancreatic cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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