|Tested species reactivity||Bovine, Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to the C-terminus of human PDZK1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody does not react with rat samples.
PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, it may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. It may also play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. PDZK1 functions to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism and may be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intestinal anion exchanger down-regulated in adenoma (DRA) is inhibited by intracellular calcium.
PA3-16818 was used in western blot to investigate the role of PDZK1 in calcium-mediated inhibition of colonic NaCl absorption
|Lamprecht G,Hsieh CJ,Lissner S,Nold L,Heil A,Gaco V,Schäfer J,Turner JR,Gregor M||The Journal of biological chemistry (284:19744)||2009|