Immunofluorescent analysis of PELO showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PELO polyclonal antibody (Product # PA5-31697) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 276 of PELO|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31697 targets PELO in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-31697 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 276 of Human PELO.
This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.