Invitrogen

PENK Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (7) PENK antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PENK Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PENK in human adrenal gland using a PENK Polyclonal Antibody (Product # PA5-53021) shows strong cytoplasmic positivity in glandular medulla cells.

PENK Antibody in Western Blot (WB) — Western blot analysis of PENK in control (vector only transfected HEK293T lysate) and PENK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PENK Polyclonal Antibody (Product # PA5-53021).

PENK Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PENK demonstrates antibody specificity. Immunohistochemical analysis of PENK using anti-PENK Polyclonal Antibody (Product # PA5-53021), shows significant staining of PENK in human adrenal gland and shows minimal or weak staining in human human kidney tissues. The relative expression levels of PENK within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-53021

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:1,000-1:2,500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PENK View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2645384

Product Specific Information

Immunogen sequence: ECSQDCATCS YRLVRPADIN FLACVMECEG KLPSLKIWET CKELLQLSKP ELPQDGTSTL RENSKPEESH LLAKRYGGFM KRYGGFMKKM DELYPMEPEE EANGSEILAK RYGGFMKKDA EE

Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 80%.

Target Information

neuropeptide hormone; plays a role in the responses to stress and pain perception.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: enkephalin A; Leu-enkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Met-enkephalin-Arg-Phe; OGF; Opioid growth factor; PENK(114-133); PENK(143-183); PENK(237-258); preproenkephalin; Proenkephalin-A; Synenkephalin

Gene Aliases: PE; PENK; PENK-A

UniProt ID: (Human) P01210

Entrez Gene ID: (Human) 5179

Molecular Function: neuropeptide