Invitrogen

PENK Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (7) PENK antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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PENK Antibody in Western Blot (WB) — Western blot analysis of PENK in control (vector only transfected HEK293T lysate) and PENK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PENK Polyclonal Antibody (Product # PA5-53021).

PENK Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PENK in human kidney using PENK Polyclonal Antibody (Product # PA5-53021) shows low expression as expected.

PENK Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of PENK demonstrates antibody specificity. Immunohistochemical analysis of PENK using anti-PENK Polyclonal Antibody (Product # PA5-53021), shows significant staining of PENK in human adrenal gland and shows minimal or weak staining in human human kidney tissues. The relative expression levels of PENK within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

1:1000-1:2500

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PENK

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2645384

Product Specific Information

Immunogen sequence: ECSQDCATCS YRLVRPADIN FLACVMECEG KLPSLKIWET CKELLQLSKP ELPQDGTSTL RENSKPEESH LLAKRYGGFM KRYGGFMKKM DELYPMEPEE EANGSEILAK RYGGFMKKDA EE

Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 80%.

Target Information

neuropeptide hormone; plays a role in the responses to stress and pain perception.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: enkephalin A; Leu-enkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Met-enkephalin-Arg-Phe; OGF; Opioid growth factor; PENK(114-133); PENK(143-183); PENK(237-258); preproenkephalin; Proenkephalin-A; Synenkephalin

Gene Aliases: PE; PENK; PENK-A

UniProt ID: (Human) P01210

Entrez Gene ID: (Human) 5179

Molecular Function: neuropeptide peptide hormone signaling molecule