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Invitrogen
PER1 Polyclonal Antibody
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FIGURE: 1 / 15
PER1 Antibody (PA1-524) in ICC/IF
Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More
Product Details
PA1-524
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Fruit fly,
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: P(39) S L A D D T D A N S N G(51) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-524 detects PER1 from human and mouse tissues as well as recombinant mouse PER1.
PA1-524 has been sucessfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~136 kDa protein representing overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with PA1-524 results in cytoplasmic staining.
PA1-524 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1. PA1-524 immunizing peptide (Cat. # PEP-076) is available for use in neutralization and control experiments.
Target Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPer; hPER1; mPER1; period circadian clock 1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of; unnamed protein product
Gene Aliases: hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI
UniProt ID: (Human) O15534, (Mouse) O35973
Entrez Gene ID: (Human) 5187, (Mouse) 18626
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
protein binding
kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
E-box binding
DNA-binding transcription factor binding
transcription factor activity, transcription factor binding
signal transducer activity
transcription factor binding
transcription cofactor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
regulation of sodium ion transport
chromatin remodeling
circadian rhythm
entrainment of circadian clock
posttranscriptional regulation of gene expression
circadian regulation of gene expression
regulation of hair cycle
regulation of circadian rhythm
negative regulation of I-kappaB kinase/NF-kappaB signaling
entrainment of circadian clock by photoperiod
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
rhythmic process
response to cAMP
regulation of cytokine production involved in inflammatory response
regulation of p38MAPK cascade
negative regulation of glucocorticoid receptor signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
signal transduction
histone H3 acetylation
histone H4 acetylation
histone H3 deacetylation
circadian regulation of translation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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