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Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Fruit fly, Hamster, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: P(39) S L A D D T D A N S N G(51) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:50-1:500|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-524 detects PER1 from human and mouse tissues as well as recombinant mouse PER1.
PA1-524 has been sucessfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~136 kDa protein representing overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with PA1-524 results in cytoplasmic staining.
PA1-524 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1. PA1-524 immunizing peptide (Cat. # PEP-076) is available for use in neutralization and control experiments.
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Modulation of mammalian circadian rhythms by tumor necrosis factor-α.
PA1-524 was used in immunohistochemistry to study the mechanism by which TNF-alpha modulates the mammalian circadian rhythm
|Paladino N,Mul Fedele ML,Duhart JM,Marpegan L,Golombek DA||Chronobiology international (31:668)||2014|
cGMP-phosphodiesterase inhibition enhances photic responses and synchronization of the biological circadian clock in rodents.
PA1-524 was used in immunohistochemistry to study the role of the cGMP pathway in the response of the rodent circadian clock to light
|Plano SA,Agostino PV,de la Iglesia HO,Golombek DA||PloS one (7:null)||2012|
Circadian entrainment to light-dark cycles involves extracellular nitric oxide communication within the suprachiasmatic nuclei.
PA1-524 was used in immunohistochemistry to investigate the mechanism of circadian entrainment to light-dark cycles
|Plano SA,Golombek DA,Chiesa JJ||The European journal of neuroscience (31:876)||2010|
Constitutive expression of the Period1 gene impairs behavioral and molecular circadian rhythms.
PA1-524 was used in immunohistochemistry to investigate the impact of period1 gene on behavioral and molecular circadian rhythms.
|Numano R,Yamazaki S,Umeda N,Samura T,Sujino M,Takahashi R,Ueda M,Mori A,Yamada K,Sakaki Y,Inouye ST,Menaker M,Tei H||Proceedings of the National Academy of Sciences of the United States of America (103:3716)||2006|
Non-cyclic and developmental stage-specific expression of circadian clock proteins during murine spermatogenesis.
PA1-524 was used in immunohistochemistry to investigate the expression of circadian clock proteins during murine spermatogenesis
|Alvarez JD,Chen D,Storer E,Sehgal A||Biology of reproduction (69:81)||2003|
Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per01) by mammalian period homologues mPer1 and mPer2.
PA1-524 was used in immunohistochemistry to investigate the role of period gene in the generation of circadian rhythm
|Shigeyoshi Y,Meyer-Bernstein E,Yagita K,Fu W,Chen Y,Takumi T,Schotland P,Sehgal A,Okamura H||Genes to cells : devoted to molecular & cellular mechanisms (7:163)||2002|
A role for the circadian clock protein Per1 in the regulation of the NaCl co-transporter (NCC) and the with-no-lysine kinase (WNK) cascade in mouse distal convoluted tubule cells.
PA1-524 was used in immunoprecipitation and western blot to study the transcriptional regulation NaCl co-transporter, WNK1 and WNK4 expression in the murine distal convoluted tubule by the Per1 clock protein
|Richards J,Ko B,All S,Cheng KY,Hoover RS,Gumz ML||The Journal of biological chemistry (289:11791)||2014|
Human epidermal stem cell function is regulated by circadian oscillations.
PA1-524 was used in western blot to study the role of circadian rhythms in modulating the function of human skin stem cells
|Janich P,Toufighi K,Solanas G,Luis NM,Minkwitz S,Serrano L,Lehner B,Benitah SA||Cell stem cell (13:745)||2013|
Opposing actions of Per1 and Cry2 in the regulation of Per1 target gene expression in the liver and kidney.
PA1-524 was used in immunoprecipitation and western blot to study the expression of Per1 target genes in liver and kidney and the opposing effects of Per1 and Cry2
|Richards J,All S,Skopis G,Cheng KY,Compton B,Srialluri N,Stow L,Jeffers LA,Gumz ML||American journal of physiology. Regulatory, integrative and comparative physiology (305:R735)||2013|
Role of Per1 and the mineralocorticoid receptor in the coordinate regulation of αENaC in renal cortical collecting duct cells.
PA1-524 was used in ChIP assay and western blot to study the mechanisms underlying the regulation of the alpha-ENaC aldosterone response by Per1 and the mineralocorticoid receptor
|Richards J,Jeffers LA,All SC,Cheng KY,Gumz ML||Frontiers in physiology (4:null)||2013|
Regulation of αENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.
PA1-524 was used in western blot to study the role of the circadian clock protein Period1 in regulating ENaC.
|Gumz ML,Cheng KY,Lynch IJ,Stow LR,Greenlee MM,Cain BD,Wingo CS||Biochimica et biophysica acta (1799:622)||2010|
Loss of melatonin signalling and its impact on circadian rhythms in mouse organs regulating blood glucose.
PA1-524 was used in western blot to investigate the importance of melatonin in circadian rhythms in mouse pancreas and liver
|Mühlbauer E,Gross E,Labucay K,Wolgast S,Peschke E||European journal of pharmacology (606:61)||2009|
Indication of circadian oscillations in the rat pancreas.
PA1-524 was used in western blot to investigate peripheral circadian oscillation in rat pancreas
|Mühlbauer E,Wolgast S,Finckh U,Peschke D,Peschke E||FEBS letters (564:91)||2004|
|Mouse||1:200 to 1:1,000||
Expression of circadian rhythm genes in gonadotropin-releasing hormone-secreting GT1-7 neurons.
PA1-524 was used in western blot to investigate the role of the clock machinery in GnRH neuronal function.
|Gillespie JM,Chan BP,Roy D,Cai F,Belsham DD||Endocrinology (144:5285)||2003|
Up-regulation of Per1 expression by estradiol and progesterone in the rat uterus.
PA1-524 was used in immunocytochemistry to study the effect of estradiol and progesterone on Per1 expression in the rat uterus
|He PJ,Hirata M,Yamauchi N,Hattori MA||The Journal of endocrinology (194:511)||2007|
Period gene expression in mouse endocrine tissues.
PA1-524 was used in immunocytochemistry to study the expression of PER1 in mouse endocrine tissues
|Bittman EL,Doherty L,Huang L,Paroskie A||American journal of physiology. Regulatory, integrative and comparative physiology (285:R561)||2003|
circadian clock protein PERIOD 1; circadian pacemaker protein RIGUI; hPER1; Period 1; period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of
hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI