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Invitrogen
PER1 Polyclonal Antibody
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FIGURE: 1 / 2
PER1 Antibody (PA5-85533) in WB
Western Blot analysis of PER1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PER1 Polyclonal Antibody (Product # PA5-85533) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-85533
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PER1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (88%), Rat (90%), Bovine (89%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPer; hPER1; per1 {ECO:0000312|RGD:727863}; period 1; period circadian clock 1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of; rPER1; unnamed protein product
Gene Aliases: hPER; KIAA0482; PER; PER1; RIGUI; rper1
UniProt ID: (Human) O15534, (Rat) Q8CHI5
Entrez Gene ID: (Human) 5187, (Rat) 287422
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
protein binding
kinase binding
chromatin DNA binding
ubiquitin protein ligase binding
E-box binding
DNA-binding transcription factor binding
transcription factor activity, transcription factor binding
signal transducer activity
transcription factor binding
transcription cofactor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
regulation of sodium ion transport
chromatin remodeling
circadian rhythm
entrainment of circadian clock
posttranscriptional regulation of gene expression
circadian regulation of gene expression
regulation of hair cycle
regulation of circadian rhythm
negative regulation of I-kappaB kinase/NF-kappaB signaling
entrainment of circadian clock by photoperiod
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
rhythmic process
response to cAMP
regulation of cytokine production involved in inflammatory response
regulation of p38MAPK cascade
negative regulation of glucocorticoid receptor signaling pathway
transcription, DNA-templated
signal transduction
histone H3 acetylation
histone H4 acetylation
histone H3 deacetylation
circadian regulation of translation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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