Rockland

PERK Polyclonal Antibody

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PERK Polyclonal Antibody

Product Details

100-401-962

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

1:1,000
-

ELISA (ELISA)

1:4,000-1:20,000
-

Immunoprecipitation (IP)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

80 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antiserum is directed against PERK and reacts with the PERK from mouse tissues. Reactivity to other species is unknown.

Target Information

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3

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Gene Aliases: AI427929; Eif2ak3; Pek; Perk

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UniProt ID: (Mouse) Q9Z2B5

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Entrez Gene ID: (Mouse) 13666

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein phosphatase binding identical protein binding Hsp90 protein binding eukaryotic initiation factor eIF2 binding non-receptor serine/threonine protein kinase
Process(es)
skeletal system development ossification angiogenesis chondrocyte development translation regulation of translation protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process ER overload response response to unfolded protein endoplasmic reticulum organization lactation positive regulation of signal transduction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression regulation of translational initiation by eIF2 alpha phosphorylation phosphorylation negative regulation of translation peptidyl-serine phosphorylation regulation of fatty acid metabolic process calcium-mediated signaling bone mineralization endoplasmic reticulum unfolded protein response pancreas development endocrine pancreas development negative regulation of myelination negative regulation of translation in response to stress positive regulation of protein binding SREBP signaling pathway response to endoplasmic reticulum stress PERK-mediated unfolded protein response cellular response to glucose starvation negative regulation of apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase I promoter protein autophosphorylation insulin-like growth factor receptor signaling pathway protein homooligomerization intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of protein localization to nucleus negative regulation of translation in response to endoplasmic reticulum stress regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of glutathione biosynthetic process positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
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