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PERK/EIF2AK3 Polyclonal Antibody

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PERK/EIF2AK3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 24390-1-AP (PERK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PERK/EIF2AK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas slide using 24390-1-AP ( PERK antibody at dilution of 1:50.

PERK/EIF2AK3 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 24390-1-AP ( PERK antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

24390-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-

Product Specifications
Species Reactivity	Human
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PERK/EIF2AK3 Fusion Protein Ag18254 (767-1116 aa encoded by BC126354) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TDCDVEDGT MDGNDEGHSF ELCPSEASPY VRSRERTSSS IVFEDSGCDN ASSKEEPKTN RLHIGNHCAN KLTAFKPTSS KSSSEATLSI SPPRPTTLSL DLTKNTTEKL QPSSPKVYLY IQMQLCRKEN LKDWMNGRCT IEERERSVCL HIFLQIAEAV EFLHSKGLMH RDLKPSNIFF TMDDVVKVGD FGLVTAMDQD EEEQTVLTPM PAYARHTGQV GTKLYMSPEQ IHGNSYSHKV DIFSLGLILF ELLYPFSTQM ERVRTLTDVR NLKFPPLFTQ KYPCEYVMVQ DMLSPSPMER PEAINIIENA VFEDLDFPGK TVLRQRSRSL SSSGTKHSRQ SNNSHSPLPS N (767-1116 aa encoded by BC126354)

Target Information

PERK, a member of the GCN2 subfamily of Ser/Thr protein kinases, phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It likely serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1 Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction Expression of this Type I membrane protein is ubiquitous, with highest levels seen in secretory tissues. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dexmedetomidine alleviates cerebral ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress dependent apoptosis through the PERK-CHOP-Caspase-11 pathway.

Brain research

Liu C,Fu Q,Mu R,Wang F,Zhou C,Zhang L,Yu B,Zhang Y,Fang T,Tian F

Sat Dec 15 00:00:00 EST 2018

Montelukast inhibits hypoxia inducible factor-1α translation in prostate cancer cells.

Cancer biology & therapy

Tang C,Lei H,Zhang J,Liu M,Jin J,Luo H,Xu H,Wu Y

Fri Aug 03 00:00:00 EDT 2018

Polysaccharide from Angelica sinensis protects H9c2 cells against oxidative injury and endoplasmic reticulum stress by activating the ATF6 pathway.

The Journal of international medical research

Niu X,Zhang J,Ling C,Bai M,Peng Y,Sun S,Li Y,Zhang Z

Tue May 01 00:00:00 EDT 2018

7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.

Marine drugs

Tsai TC,Lai KH,Su JH,Wu YJ,Sheu JH

Mon Mar 26 00:00:00 EDT 2018

Transthyretin Exerts Pro-Apoptotic Effects in Human Retinal Microvascular Endothelial Cells Through a GRP78-Dependent Pathway in Diabetic Retinopathy.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Shao J,Yin Y,Yin X,Ji L,Xin Y,Zou J,Yao Y

Tue Oct 31 00:00:00 EDT 2017

PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.

Experimental and therapeutic medicine

Xu X,Cui Y,Cao L,Zhang Y,Yin Y,Hu X

Mon May 01 00:00:00 EDT 2017

Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release.

Chemical research in toxicology

Xu D,Su C,Song X,Shi Q,Fu J,Hu L,Xia X,Song E,Song Y

Mon Jun 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase

Gene Aliases: EIF2AK3; PEK; PERK; WRS

UniProt ID: (Human) Q9NZJ5

Entrez Gene ID: (Human) 9451

Molecular Function: non-receptor serine/threonine protein kinase

Function(s)

skeletal system development ossification angiogenesis chondrocyte development protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process ER overload response endoplasmic reticulum organization positive regulation of vascular endothelial growth factor production positive regulation of gene expression regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation peptidyl-serine phosphorylation calcium-mediated signaling insulin secretion bone mineralization endoplasmic reticulum unfolded protein response endocrine pancreas development negative regulation of myelination negative regulation of translational initiation in response to stress cellular response to amino acid starvation response to endoplasmic reticulum stress eiF2alpha phosphorylation in response to endoplasmic reticulum stress PERK-mediated unfolded protein response cellular response to glucose starvation positive regulation of transcription from RNA polymerase I promoter protein autophosphorylation insulin-like growth factor receptor signaling pathway protein homooligomerization regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation cellular response to cold positive regulation of protein localization to nucleus regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to manganese-induced endoplasmic reticulum stress

Process(es)

protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein binding ATP binding enzyme binding protein phosphatase binding identical protein binding protein homodimerization activity Hsp90 protein binding

PERK/EIF2AK3 Polyclonal Antibody

Product Details

24390-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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