Proteintech

PEX14 Polyclonal Antibody

Product Image Gallery

PEX14 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10594-1-AP (PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PEX14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

PEX14 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PEX14 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with .2ug PEX14 antibody (10594-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

PEX14 Antibody in Immunoprecipitation (IP) — IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:2000-1:10,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Artificial Control, Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PEX14 Fusion Protein Ag0932

Conjugate

Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa and recognize peroxisomal structures in human, monkey and mouse cells.

Target Information

PEX14 belongs to the peroxin 14 family. It is a component of the peroxisomal translocation machinery with PEX13 and PEX17 and interacts with both the PTS1 and PTS2 receptors. PEX14 binds directly to PEX17. Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) and Zellweger syndrome (ZWS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: NF-E2 associated polypeptide 2; Peroxin 14; Peroxin-14; Peroxisomal membrane anchor protein PEX14; peroxisomal membrane anchor protein Pex14p; Peroxisomal membrane protein PEX14; peroxisomal protein Pex14; PEXE; PTS1 receptor docking protein; PTS1 receptor-docking protein

Gene Aliases: dJ734G22.2; NAPP2; PBD13A; PEX14; Pex14p; R75137

UniProt ID: (Human) O75381, (Mouse) Q9R0A0, (Rat) Q642G4

Entrez Gene ID: (Human) 5195, (Mouse) 56273, (Rat) 64460

Molecular Function: receptor