Proteintech

PEX14 Polyclonal Antibody

Product Image Gallery

PEX14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10594-1-AP (PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PEX14 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

PEX14 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PEX14 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with .2ug PEX14 antibody (10594-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

PEX14 Antibody in Immunoprecipitation (IP) — IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Product Details

10594-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 14 publications 14 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PEX14 Fusion Protein Ag0932 (77-377 aa encoded by BC006327) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa and recognize peroxisomal structures in human, monkey and mouse cells.

Immunogen sequence: PSSLGPATQ VVPVQPPHLI SQPYSPAGSR WRDYGALAII MAGIAFGFHQ LYKKYLLPLI LGGREDRKQL ERMEAGLSEL SGSVAQTVTQ LQTTLASVQE LLIQQQQKIQ ELAHELAAAK ATTSTNWILE SQNINELKSE INSLKGLLLN RRQFPPSPSA PKIPSWQIPV KSPSPSSPAA VNHHSSSDIS PVSNESTSSS PGKEGHSPEG STVTYHLLGP QEEGEGVVDV KGQVRMEVQG EEEKREDKED EEDEEDDDVS HVDEEDCLGV QREDRRGGDG QINEQVEKLR RPEGASNESE RD (77-377 aa encoded by BC006327)

Target Information

PEX14 belongs to the peroxin 14 family. It is a component of the peroxisomal translocation machinery with PEX13 and PEX17 and interacts with both the PTS1 and PTS2 receptors. PEX14 binds directly to PEX17. Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) and Zellweger syndrome (ZWS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

Scientific reports

Yu Z,Ma J,Li X,Liu Y,Li M,Wang L,Zhao M,He H,Zhang Y,Rao Q,Zhao D,Wang Y,Fan L,Li P,Liu Y,Liu F,Zhang F,Ye J,Yan Q,Guo S,Wang Z

Mon Jul 02 00:00:00 EDT 2018

Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.

Scientific reports

Soliman K,Göttfert F,Rosewich H,Thoms S,Gärtner J

Thu May 17 00:00:00 EDT 2018

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Methods in molecular biology (Clifton, N.J.)

Schönenberger MJ,Kovacs WJ

Thu May 18 00:00:00 EDT 2017

Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy.

Journal of cell science

Hofhuis J,Bersch K,Büssenschütt R,Drzymalski M,Liebetanz D,Nikolaev VO,Wagner S,Maier LS,Gärtner J,Klinge L,Thoms S

Wed Mar 01 00:00:00 EST 2017

Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5-dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics.

Molecular biology of the cell

Cherok E,Xu S,Li S,Das S,Meltzer WA,Zalzman M,Wang C,Karbowski M

Wed Feb 01 00:00:00 EST 2017

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.

European journal of human genetics : EJHG

Smith CE,Poulter JA,Levin AV,Capasso JE,Price S,Ben-Yosef T,Sharony R,Newman WG,Shore RC,Brookes SJ,Mighell AJ,Inglehearn CF

Tue Nov 01 00:00:00 EDT 2016

The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.

Open biology

Hofhuis J,Schueren F,Nötzel C,Lingner T,Gärtner J,Jahn O,Thoms S

Tue Nov 01 00:00:00 EDT 2016

A Dendritic Golgi Satellite between ERGIC and Retromer.

Cell reports

Mikhaylova M,Bera S,Kobler O,Frischknecht R,Kreutz MR

Tue Jan 12 00:00:00 EST 2016

Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins.

Scientific reports

Buentzel J,Vilardi F,Lotz-Havla A,Gärtner J,Thoms S

Wed Dec 02 00:00:00 EST 2015

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Cell metabolism

Walter KM,Schönenberger MJ,Trötzmüller M,Horn M,Elsässer HP,Moser AB,Lucas MS,Schwarz T,Gerber PA,Faust PL,Moch H,Köfeler HC,Krek W,Kovacs WJ

Tue Nov 04 00:00:00 EST 2014

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Cell metabolism

Walter KM,Schönenberger MJ,Trötzmüller M,Horn M,Elsässer HP,Moser AB,Lucas MS,Schwarz T,Gerber PA,Faust PL,Moch H,Köfeler HC,Krek W,Kovacs WJ

Tue Nov 04 00:00:00 EST 2014

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.

eLife

Schueren F,Lingner T,George R,Hofhuis J,Dickel C,Gärtner J,Thoms S

Tue Sep 23 00:00:00 EDT 2014

Peroxisomes are juxtaposed to strategic sites on mitochondria.

Molecular bioSystems

Cohen Y,Klug YA,Dimitrov L,Erez Z,Chuartzman SG,Elinger D,Yofe I,Soliman K,Gärtner J,Thoms S,Schekman R,Elbaz-Alon Y,Zalckvar E,Schuldiner M

Tue Jul 01 00:00:00 EDT 2014

Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.

Human molecular genetics

Krause C,Rosewich H,Woehler A,Gärtner J

Tue Oct 01 00:00:00 EDT 2013

Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.

Human molecular genetics

Krause C,Rosewich H,Woehler A,Gärtner J

Tue Oct 01 00:00:00 EDT 2013

Charcot-Marie-Tooth disease-associated mutants of GDAP1 dissociate its roles in peroxisomal and mitochondrial fission.

EMBO reports

Huber N,Guimaraes S,Schrader M,Suter U,Niemann A

Sat Jun 01 00:00:00 EDT 2013

Lymphoblastoid cell lines for diagnosis of peroxisome biogenesis disorders.

JIMD reports

Grønborg S,Krätzner R,Rosewich H,Gärtner J

Mon Feb 25 00:00:00 EST 2013

Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.

Biochimica et biophysica acta

Kovacs WJ,Charles KN,Walter KM,Shackelford JE,Wikander TM,Richards MJ,Fliesler SJ,Krisans SK,Faust PL

Fri Jun 01 00:00:00 EDT 2012

Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.

Biochimica et biophysica acta

Kovacs WJ,Charles KN,Walter KM,Shackelford JE,Wikander TM,Richards MJ,Fliesler SJ,Krisans SK,Faust PL

Fri Jun 01 00:00:00 EDT 2012

The unusual extended C-terminal helix of the peroxisomal α/β-hydrolase Lpx1 is involved in dimer contacts but dispensable for dimerization.

Journal of structural biology

Thoms S,Hofhuis J,Thöing C,Gärtner J,Niemann HH

Thu Sep 01 00:00:00 EDT 2011

A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity.

FEBS letters

Kassmann CM,Quintes S,Rietdorf J,Möbius W,Sereda MW,Nientiedt T,Saher G,Baes M,Nave KA

Thu Jul 21 00:00:00 EDT 2011

A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity.

FEBS letters

Kassmann CM,Quintes S,Rietdorf J,Möbius W,Sereda MW,Nientiedt T,Saher G,Baes M,Nave KA

Thu Jul 21 00:00:00 EDT 2011

Typical cMRI pattern as diagnostic clue for D-bifunctional protein deficiency without apparent biochemical abnormalities in plasma.

American journal of medical genetics. Part A

Grønborg S,Krätzner R,Spiegler J,Ferdinandusse S,Wanders RJ,Waterham HR,Gärtner J

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: NF-E2 associated polypeptide 2; Peroxin 14; Peroxin-14; Peroxisomal membrane anchor protein PEX14; peroxisomal membrane anchor protein Pex14p; Peroxisomal membrane protein PEX14; peroxisomal protein Pex14; PEXE; PTS1 receptor docking protein; PTS1 receptor-docking protein

Gene Aliases: dJ734G22.2; NAPP2; PBD13A; PEX14; Pex14p; R75137

UniProt ID: (Human) O75381, (Mouse) Q9R0A0, (Rat) Q642G4

Entrez Gene ID: (Human) 5195, (Mouse) 56273, (Rat) 64460

Molecular Function: primary active transporter