Immunofluorescent staining of PEX5 in human cell line A-431 using a PEX5 Polyclonal Antibody (Product # PA5-58717) shows localization to cytosol and the Golgi apparatus.

PEX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PEX5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PEX5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS were transfected with, control siRNA, PEX5 siRNA (probe #1), and PEX5 siRNA (probe #2) and a decrease in signal intensity was observed in Western Blot application using anti-PEX5 Polyclonal Antibody (Product # PA5-58717) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Immunogen image

Immunogen Image PEX5 Polyclonal Antibody

Product Details

PA5-58717

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PEX5. Recombinant protein control fragment (Product #RP-102867).

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

10 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2645412

Product Specific Information

Immunogen sequence: RAQAEQWAAE FIQQQGTSDA WVDQFTRPVN TSALDMEFER AKSAIESDVD FWDKLQAELE EMAKRDAEAH PWLSDYDDLT SATYDKGYQF EEEN

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 92%.

Target Information

The product of this gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Peroxin-5; Peroxisomal C-terminal targeting signal import receptor; peroxisomal import receptor 5; peroxisomal targeting signal 1 (SKL type) receptor; Peroxisomal targeting signal 1 receptor; peroxisomal targeting signal import receptor; peroxisomal targeting signal receptor 1; Peroxisome receptor 1; PTS1 BP; PTS1 receptor; PTS1-BP; unnamed protein product

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Gene Aliases: PBD2A; PBD2B; PEX5; PTS1-BP; PTS1R; PXR1; RCDP5

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UniProt ID: (Human) P50542

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Entrez Gene ID: (Human) 5830

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Function(s)

peroxisome targeting sequence binding peroxisome matrix targeting signal-1 binding protein binding enzyme binding small GTPase binding peroxisome membrane targeting sequence binding protein carrier chaperone

Process(es)

very long-chain fatty acid metabolic process pexophagy neuron migration protein monoubiquitination protein targeting to peroxisome lipid metabolic process fatty acid beta-oxidation mitochondrion organization mitochondrial membrane organization endoplasmic reticulum organization peroxisome organization protein transport protein import into peroxisome matrix protein import into peroxisome matrix, docking protein import into peroxisome matrix, translocation protein import into peroxisome matrix, receptor recycling cerebral cortex cell migration cerebral cortex neuron differentiation negative regulation of protein complex assembly cellular response to reactive oxygen species positive regulation of multicellular organism growth protein unfolding protein import into peroxisome matrix, substrate release protein import into peroxisome membrane cell development neuromuscular process protein tetramerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PEX5 Polyclonal Antibody

PEX5 Antibody (PA5-58717) in ICC/IF

Product Details

PA5-58717

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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