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PF4 Antibody (500-P05BT-500UG) in WB
Western Blot: To detect hPF-4 by Western Blot analysis PF4 Polyclonal Antibody, Biotin (Product # 500-P05BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P05BT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 7.8 kDa Recombinant Human PF-4 (CXCL4)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human PF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PF-4 (500-P05) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PF-4.
Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P05BT-1MG will be provided as 2 x 500 µg
Target Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Iroplact; Oncostatin-A; PF-4; Platelet factor 4
Gene Aliases: CXCL4; PF-4; PF4; SCYB4
UniProt ID: (Human) P02776
Entrez Gene ID: (Human) 5196
platelet degranulation
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
negative regulation of angiogenesis
cytokine-mediated signaling pathway
platelet activation
leukocyte chemotaxis
positive regulation of cAMP metabolic process
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of MHC class II biosynthetic process
positive regulation of macrophage differentiation
negative regulation of megakaryocyte differentiation
negative regulation of cytolysis
positive regulation of transcription from RNA polymerase II promoter
chemokine-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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