Invitrogen

PF4 Polyclonal Antibody

Product Image Gallery

PF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PF4 on paraffin-embedded human lung cancer tissue. Sample was incubated with PF4 polyclonal antibody (Product# PA5-79808).

PF4 Antibody in Western Blot (WB) — Western blot analysis of PF4 in Lane 1: recombinant human CXCL4 protein, Lane 2: recombinant human CXCL4 protein, Lane 3: recombinant human CXCL4 protein using 10, 5 and 2.5 ng, respectively per well. Sample was incubated with PF4 (Product # PA5-79808).

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PF4.

Conjugate

Unconjugated

Form

Lyophilized

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

RRID

AB_2746923

Product Specific Information

The synthetic peptide sequence is 85-101aa, DLQAPLYKKIIKKLLES

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Endothelial cell growth inhibitor; Iroplact; Oncostatin-A; PF-4; Platelet factor 4; Platelet factor 4, short form; short form

Gene Aliases: CXCL4; PF-4; PF4; SCYB4

UniProt ID: (Human) P02776

Entrez Gene ID: (Human) 5196

Molecular Function: chemokine cytokine signaling molecule