Invitrogen

PF4 Polyclonal Antibody

View all (16) PF4 antibodies

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PF4 Antibody in Western Blot (WB) — Western blot analysis of PF4 in mouse skeletal muscle extract (lane 1). Sample was incubated with PF4 polyclonal antibody (Product # PA5-79811) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

PF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PF4 on paraffin-embedded mouse spleen tissue. Sample was incubated with PF4 polyclonal antibody (Product# PA5-79811) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

PF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PF4 on paraffin-embedded rat spleen tissue. Sample was incubated with PF4 polyclonal antibody (Product# PA5-79811) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

-

ELISA (ELISA)

0.1-0.5 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived mouse PF4 recombinant protein (Position: V30-S105).

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2746926

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; PF-4; Platelet factor 4

Gene Aliases: Cxcl4; PF-4; Pf4; Pf4a; RATPF4A; Scyb4

UniProt ID: (Rat) P06765, (Mouse) Q9Z126

Entrez Gene ID: (Rat) 360918, (Mouse) 56744

Molecular Function: chemokine cytokine signaling molecule

positive regulation of leukocyte chemotaxis protein complex assembly inflammatory response immune response G-protein coupled receptor signaling pathway positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation negative regulation of angiogenesis cytokine-mediated signaling pathway platelet activation leukocyte chemotaxis positive regulation of cAMP metabolic process response to lipopolysaccharide positive regulation of tumor necrosis factor production regulation of cell proliferation positive regulation of cAMP-mediated signaling negative regulation of MHC class II biosynthetic process positive regulation of macrophage differentiation negative regulation of megakaryocyte differentiation negative regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter chemokine-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand chemotaxis cell chemotaxis

Process(es)

molecular_function chemokine activity heparin binding CXCR chemokine receptor binding CXCR3 chemokine receptor binding cytokine activity