Proteintech

PFKFB3 Polyclonal Antibody

Product Image Gallery

PFKFB3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

PFKFB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PFKFB3 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PFKFB3 Antibody in Immunoprecipitation (IP) — IP result of anti-PFKFB3-Specific (IP:13763-1-AP, 4 ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.

Product Details

13763-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 20 publications 20 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PFKFB3-Specific Fusion Protein Ag4744 (243-520 aa encoded by BC040482) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to PFKFB3.

Immunogen sequence: HVQPRTIYL CRHGENEHNL QGRIGGDSGL SSRGKKFASA LSKFVEEQNL KDLRVWTSQL KSTIQTAEAL RLPYEQWKAL NEIDAGVCEE LTYEEIRDTY PEEYALREQD KYYYRYPTGE SYQDLVQRLE PVIMELERQE NVLVICHQAV LRCLLAYFLD KSAEEMPYLK CPLHTVLKLT PVAYGCRVES IYLNVESVCT HRERSEDAKK GPNPLMRRNS VTPLASPEPT KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQNMKGSR SSADSSRKH (243-520 aa encoded by BC040482)

Target Information

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Glycolytic Enzyme PFKFB3 Controls TNF-α-Induced Endothelial Proinflammatory Responses.

Inflammation

Zhang R,Li R,Liu Y,Li L,Tang Y

Fri Feb 01 00:00:00 EST 2019

Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination.

Nature communications

Gustafsson NMS,Färnegårdh K,Bonagas N,Ninou AH,Groth P,Wiita E,Jönsson M,Hallberg K,Lehto J,Pennisi R,Martinsson J,Norström C,Hollers J,Schultz J,Andersson M,Markova N,Marttila P,Kim B,Norin M,Olin T,Helleday T

Mon Sep 24 00:00:00 EDT 2018

Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination.

Nature communications

Mon Sep 24 00:00:00 EDT 2018

Exercise-Induced Changes in Glucose Metabolism Promote Physiological Cardiac Growth.

Circulation

Gibb AA,Epstein PN,Uchida S,Zheng Y,McNally LA,Obal D,Katragadda K,Trainor P,Conklin DJ,Brittian KR,Tseng MT,Wang J,Jones SP,Bhatnagar A,Hill BG

Tue Nov 28 00:00:00 EST 2017

Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis.

Nature communications

Liu Z,Yan S,Wang J,Xu Y,Wang Y,Zhang S,Xu X,Yang Q,Zeng X,Zhou Y,Gu X,Lu S,Fu Z,Fulton DJ,Weintraub NL,Caldwell RB,Zhang W,Wu C,Liu XL,Chen JF,Ahmad A,Kaddour-Djebbar I,Al-Shabrawey M,Li Q,Jiang X,Sun Y,Sodhi A,Smith L,Hong M,Huo Y

Tue Sep 19 00:00:00 EDT 2017

FGF-dependent metabolic control of vascular development.

Nature

Yu P,Wilhelm K,Dubrac A,Tung JK,Alves TC,Fang JS,Xie Y,Zhu J,Chen Z,De Smet F,Zhang J,Jin SW,Sun L,Sun H,Kibbey RG,Hirschi KK,Hay N,Carmeliet P,Chittenden TW,Eichmann A,Potente M,Simons M

Thu May 11 00:00:00 EDT 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for transforming growth factor β1-enhanced invasion of Panc1 cells in vitro.

Biochemical and biophysical research communications

Yalcin A,Solakoglu TH,Ozcan SC,Guzel S,Peker S,Celikler S,Balaban BD,Sevinc E,Gurpinar Y,Chesney JA

Sat Mar 11 00:00:00 EST 2017

p53 coordinates DNA repair with nucleotide synthesis by suppressing PFKFB3 expression and promoting the pentose phosphate pathway.

Scientific reports

Franklin DA,He Y,Leslie PL,Tikunov AP,Fenger N,Macdonald JM,Zhang Y

Wed Nov 30 00:00:00 EST 2016

Inhibition of 6-phosphofructo-2-kinase (PFKFB3) suppresses glucose metabolism and the growth of HER2+ breast cancer.

Breast cancer research and treatment

O'Neal J,Clem A,Reynolds L,Dougherty S,Imbert-Fernandez Y,Telang S,Chesney J,Clem BF

Tue Nov 01 00:00:00 EDT 2016

Inhibition of 6-phosphofructo-2-kinase (PFKFB3) suppresses glucose metabolism and the growth of HER2+ breast cancer.

Breast cancer research and treatment

O'Neal J,Clem A,Reynolds L,Dougherty S,Imbert-Fernandez Y,Telang S,Chesney J,Clem BF

Tue Nov 01 00:00:00 EDT 2016

PFKFB3-Driven Macrophage Glycolytic Metabolism Is a Crucial Component of Innate Antiviral Defense.

Journal of immunology (Baltimore, Md. : 1950)

Jiang H,Shi H,Sun M,Wang Y,Meng Q,Guo P,Cao Y,Chen J,Gao X,Li E,Liu J

Sat Oct 01 00:00:00 EDT 2016

Metabolic control of the cell cycle.

Cell cycle (Georgetown, Tex.)

Kalucka J,Missiaen R,Georgiadou M,Schoors S,Lange C,De Bock K,Dewerchin M,Carmeliet P

Mon Aug 29 00:00:00 EDT 2016

Glucose and Palmitate Differentially Regulate PFKFB3/iPFK2 and Inflammatory Responses in Mouse Intestinal Epithelial Cells.

Scientific reports

Botchlett R,Li H,Guo X,Qi T,Zhao J,Zheng J,Woo SL,Pei Y,Liu M,Hu X,Chen G,Guo T,Yang S,Li Q,Xiao X,Huo Y,Wu C

Fri Jul 08 00:00:00 EDT 2016

Type 2 Diabetes Dysregulates Glucose Metabolism in Cardiac Progenitor Cells.

The Journal of biological chemistry

Salabei JK,Lorkiewicz PK,Mehra P,Gibb AA,Haberzettl P,Hong KU,Wei X,Zhang X,Li Q,Wysoczynski M,Bolli R,Bhatnagar A,Hill BG

Fri Jun 24 00:00:00 EDT 2016

Glycolytic Reprogramming in Myofibroblast Differentiation and Lung Fibrosis.

American journal of respiratory and critical care medicine

Xie N,Tan Z,Banerjee S,Cui H,Ge J,Liu RM,Bernard K,Thannickal VJ,Liu G

Tue Dec 15 00:00:00 EST 2015

Glycolytic Reprogramming in Myofibroblast Differentiation and Lung Fibrosis.

American journal of respiratory and critical care medicine

Xie N,Tan Z,Banerjee S,Cui H,Ge J,Liu RM,Bernard K,Thannickal VJ,Liu G

Tue Dec 15 00:00:00 EST 2015

miR-26b inhibits proliferation, migration, invasion and apoptosis induction via the downregulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 driven glycolysis in osteosarcoma cells.

Oncology reports

Du JY,Wang LF,Wang Q,Yu LD

Wed Apr 01 00:00:00 EDT 2015

The monocarboxylate transporter 4 is required for glycolytic reprogramming and inflammatory response in macrophages.

The Journal of biological chemistry

Tan Z,Xie N,Banerjee S,Cui H,Fu M,Thannickal VJ,Liu G

Fri Jan 02 00:00:00 EST 2015

6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27.

Cell death & disease

Yalcin A,Clem BF,Imbert-Fernandez Y,Ozcan SC,Peker S,O'Neal J,Klarer AC,Clem AL,Telang S,Chesney J

Thu Jul 17 00:00:00 EDT 2014

Endothelial PFKFB3 plays a critical role in angiogenesis.

Arteriosclerosis, thrombosis, and vascular biology

Xu Y,An X,Guo X,Habtetsion TG,Wang Y,Xu X,Kandala S,Li Q,Li H,Zhang C,Caldwell RB,Fulton DJ,Su Y,Hoda MN,Zhou G,Wu C,Huo Y

Sun Jun 01 00:00:00 EDT 2014

Estradiol stimulates glucose metabolism via 6-phosphofructo-2-kinase (PFKFB3).

The Journal of biological chemistry

Imbert-Fernandez Y,Clem BF,O'Neal J,Kerr DA,Spaulding R,Lanceta L,Clem AL,Telang S,Chesney J

Fri Mar 28 00:00:00 EDT 2014

Estradiol stimulates glucose metabolism via 6-phosphofructo-2-kinase (PFKFB3).

The Journal of biological chemistry

Imbert-Fernandez Y,Clem BF,O'Neal J,Kerr DA,Spaulding R,Lanceta L,Clem AL,Telang S,Chesney J

Fri Mar 28 00:00:00 EDT 2014

Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism.

Cancer & metabolism

Klarer AC,O'Neal J,Imbert-Fernandez Y,Clem A,Ellis SR,Clark J,Clem B,Chesney J,Telang S

Thu Jan 23 00:00:00 EST 2014

Role of PFKFB3-driven glycolysis in vessel sprouting.

Cell

De Bock K,Georgiadou M,Schoors S,Kuchnio A,Wong BW,Cantelmo AR,Quaegebeur A,Ghesquière B,Cauwenberghs S,Eelen G,Phng LK,Betz I,Tembuyser B,Brepoels K,Welti J,Geudens I,Segura I,Cruys B,Bifari F,Decimo I,Blanco R,Wyns S,Vangindertael J,Rocha S,Collins RT,Munck S,Daelemans D,Imamura H,Devlieger R,Rider M,Van Veldhoven PP,Schuit F,Bartrons R,Hofkens J,Fraisl P,Telang S,Deberardinis RJ,Schoonjans L,Vinckier S,Chesney J,Gerhardt H,Dewerchin M,Carmeliet P

Thu Aug 01 00:00:00 EDT 2013

Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.

Journal of translational medicine

Telang S,Clem BF,Klarer AC,Clem AL,Trent JO,Bucala R,Chesney J

Wed May 16 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 6-biphosphatase 3; 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; 6PF-2-K/Fru-2,6-P2ASE brain-type isozyme; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Fructose-2; Fructose-2,6-bisphosphatase; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; inducible 6-phosphofructo-2-kinase; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; iPFK-2; PFK/FBPase 3; RB2K; Renal carcinoma antigen NY-REN-56; RP11-298K24.3

Gene Aliases: E330010H22Rik; iPFK-2; IPFK2; PFK2; PFKFB3; uPFK-2

UniProt ID: (Human) Q16875, (Rat) Q9QWQ6

Entrez Gene ID: (Human) 5209, (Rat) 117276, (Mouse) 170768

Molecular Function: carbohydrate phosphatase