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Invitrogen
PFKL Polyclonal Antibody
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FIGURE: 1 / 3
PFKL Antibody (PA5-101088) in IHC (P)
Immunohistochemistry analysis of PFKL in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PFKL polyclonal antibody (Product # PA5-101088) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-101088
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PFKL(Accession P17858), corresponding to amino acid residues P704-Y754.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total K6PL.
Target Information
Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}; ATP-PFK; ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}; EC 2.7.1.11; K6PL; liver-type 1-phosphofructokinase; PFK-B; Phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver, B-type; Phosphohexokinase; phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}
Gene Aliases: AA407869; ATP-PFK; PFK-B; PFK-L; Pfkb; PFKL
UniProt ID: (Human) P17858, (Mouse) P12382, (Rat) P30835
Entrez Gene ID: (Human) 5211, (Mouse) 18641, (Rat) 25741
6-phosphofructokinase activity
protein binding
ATP binding
kinase binding
identical protein binding
metal ion binding
fructose binding
fructose-6-phosphate binding
nucleotide binding
catalytic activity
kinase activity
transferase activity
monosaccharide binding
fructose-2,6-bisphosphate 2-phosphatase activity
carbohydrate kinase
kinase
transferase
metabolite interconversion enzyme
fructose 6-phosphate metabolic process
glycolytic process
response to glucose
fructose 1,6-bisphosphate metabolic process
negative regulation of insulin secretion
protein oligomerization
protein homotetramerization
canonical glycolysis
carbohydrate metabolic process
metabolic process
phosphorylation
carbohydrate phosphorylation
dephosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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