Proteintech

PGAM1 Polyclonal Antibody

Product Image Gallery

PGAM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PGAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 40x lens).

PGAM1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16126-1-AP (PGAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

16126-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PGAM1 Fusion Protein Ag9110 (1-254 aa encoded by BC011678) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to PGAM1.

Immunogen sequence: MAAYKLVLI RHGESAWNLE NRFSGWYDAD LSPAGHEEAK RGGQALRDAG YEFDICFTSV QKRAIRTLWT VLDAIDQMWL PVVRTWRLNE RHYGGLTGLN KAETAAKHGE AQVKIWRRSY DVPPPPMEPD HPFYSNISKD RRYADLTEDQ LPSCESLKDT IARALPFWNE EIVPQIKEGK RVLIAAHGNS LRGIVKHLEG LSEEAIMELN LPTGIPIVYE LDKNLKPIKP MQFLGDEETV RKAMEAVAAQ GKAKK (1-254 aa encoded by BC011678)

Target Information

PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 EDT 2018

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The EMBO journal

Wang L,Zhang T,Wang L,Cai Y,Zhong X,He X,Hu L,Tian S,Wu M,Hui L,Zhang H,Gao P

Mon May 15 00:00:00 EDT 2017

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: BPG-dependent PGAM 1; epididymis secretory protein Li 35; PGAM-B; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B; RP11-452K12.8

Gene Aliases: 2310050F24Rik; CDABP0006; HEL-S-35; Pgam-1; PGAM-B; PGAM1; PGAMA; Pgmut

UniProt ID: (Human) P18669, (Rat) P25113, (Mouse) Q9DBJ1

Entrez Gene ID: (Human) 5223, (Rat) 24642, (Mouse) 18648

Molecular Function: mutase