Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
PGAM1 Fusion Protein Ag9110 (1-254 aa encoded by BC011678) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.13 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
This antibody is specific to PGAM1.
Immunogen sequence: MAAYKLVLI RHGESAWNLE NRFSGWYDAD LSPAGHEEAK RGGQALRDAG YEFDICFTSV QKRAIRTLWT VLDAIDQMWL PVVRTWRLNE RHYGGLTGLN KAETAAKHGE AQVKIWRRSY DVPPPPMEPD HPFYSNISKD RRYADLTEDQ LPSCESLKDT IARALPFWNE EIVPQIKEGK RVLIAAHGNS LRGIVKHLEG LSEEAIMELN LPTGIPIVYE LDKNLKPIKP MQFLGDEETV RKAMEAVAAQ GKAKK (1-254 aa encoded by BC011678)
PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BPG-dependent PGAM 1; epididymis secretory protein Li 35; PGAM-B; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B; RP11-452K12.8
Gene Aliases: 2310050F24Rik; CDABP0006; HEL-S-35; Pgam-1; PGAM-B; PGAM1; PGAMA; Pgmut
UniProt ID: (Human) P18669, (Rat) P25113, (Mouse) Q9DBJ1
Entrez Gene ID: (Human) 5223, (Rat) 24642, (Mouse) 18648
Molecular Function:
mutase
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