Proteintech

PGAM1 Polyclonal Antibody

Product Image Gallery

PGAM1 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 16126-1-AP (PGAM1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

PGAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 (under 40x lens).

PGAM1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PGAM1 Fusion Protein Ag9110

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to PGAM1.

Target Information

PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BPG-dependent PGAM 1; epididymis secretory protein Li 35; PGAM-B; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B; RP11-452K12.8

Gene Aliases: 2310050F24Rik; CDABP0006; HEL-S-35; Pgam-1; PGAM-B; PGAM1; PGAMA; Pgmut

UniProt ID: (Human) P18669, (Rat) P25113, (Mouse) Q9DBJ1

Entrez Gene ID: (Human) 5223, (Rat) 24642, (Mouse) 18648

Function(s)

gluconeogenesis glycolytic process regulation of glycolytic process dephosphorylation regulation of pentose-phosphate shunt respiratory burst canonical glycolysis metabolic process

Process(es)

bisphosphoglycerate mutase activity bisphosphoglycerate 2-phosphatase activity phosphoglycerate mutase activity protein binding protein kinase binding 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity catalytic activity hydrolase activity isomerase activity intramolecular transferase activity, phosphotransferases