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Invitrogen
PGAM1 Monoclonal Antibody (GT256)
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FIGURE: 1 / 5
PGAM1 Antibody (MA5-27770) in ICC/IF
PGAM1 Monoclonal Antibody (GT256) detects PGAM1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% PFA for 15 min. Green: PGAM1 protein stained by PGAM1 Monoclonal Antibody (GT256) (Product # MA5-27770) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
MA5-27770
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT256
Immunogen
Recombinant protein encompassing a sequence within the center region of human PGAM1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2, mouse brain, rat brain
Predicted Reactivity: Xenopus laevis (84%), Chicken (92%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (99%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BPG-dependent PGAM 1; epididymis secretory protein Li 35; PGAM-B; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B; RP11-452K12.8
Gene Aliases: 2310050F24Rik; CDABP0006; HEL-S-35; Pgam-1; PGAM-B; PGAM1; PGAMA; Pgmut
UniProt ID: (Human) P18669, (Rat) P25113, (Mouse) Q9DBJ1
Entrez Gene ID: (Human) 5223, (Rat) 24642, (Mouse) 18648
bisphosphoglycerate mutase activity
bisphosphoglycerate 2-phosphatase activity
phosphoglycerate mutase activity
protein binding
protein kinase binding
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity
catalytic activity
hydrolase activity
isomerase activity
intramolecular transferase activity, phosphotransferases
mutase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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