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          • Primary Antibodies ›
          • PGF Antibodies

          Invitrogen

          PGF Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (29) PGF antibodies

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          Cite PGF Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          PGF Antibody in Western Blot (WB)
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          PGF Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          PGF Antibody (PA5-79814) in WB

          Western blot analysis of PGF in Lane 1: rat gastric tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse gastric tissue lysate, Lane 4: mouse liver tissue lysate, Lane 5: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PGF Antibody in Western Blot (WB)
          PGF Antibody in Western Blot (WB)
          PGF Polyclonal Antibody

          Product Details

          PA5-79814

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived rat PGF recombinant protein (Position: A24-L158).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2746929

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: rat gaster tissue, rat liver tissue, mouse gaster tissue, mouse liver tissue, mouse HEPA1-6 whole cell.

          Target Information

          Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor affects different cell types and regulates various biological responses. The activity of vascular endothelial growth factor (VEGF), which is the most potent of all angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in human placenta.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Placenta growth factor; PlGF; PlGF 1; SHGC

          View more View less

          Gene Aliases: AI854365; Pgf; PIGF; Plgf

          View more View less

          UniProt ID: (Mouse) P49764, (Rat) Q63434

          View more View less

          Entrez Gene ID: (Mouse) 18654, (Rat) 94203

          View more View less

          Function(s)
          growth factor activity protein homodimerization activity protein heterodimerization activity vascular endothelial growth factor receptor binding growth factor
          Process(es)
          angiogenesis branching involved in ureteric bud morphogenesis positive regulation of endothelial cell proliferation sprouting angiogenesis multicellular organism development positive regulation of cell proliferation cell differentiation positive regulation of angiogenesis positive regulation of cell division regulation of morphogenesis of a branching structure response to hypoxia female pregnancy organ regeneration cellular response to hormone stimulus response to drug
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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