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Invitrogen
PGP9.5 Polyclonal Antibody
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FIGURE: 1 / 7
PGP9.5 Antibody (PA1-20158) in ICC/IF
Immunofluorescence analysis of PGP9.5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PGP9.5 Rabbit Polyclonal Antibody (Product # PA1-20158) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA1-20158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
ASSEDTLLKDAAKVCR
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Human brain lysate.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase 1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1; UCHL
Gene Aliases: AW822034; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1
UniProt ID: (Pig) Q6SEG5, (Human) P09936, (Mouse) Q9R0P9, (Rat) Q00981
Entrez Gene ID: (Pig) 396637, (Human) 7345, (Mouse) 22223, (Rat) 29545
cysteine-type endopeptidase activity
ligase activity
ubiquitin protein ligase binding
alpha-2A adrenergic receptor binding
ubiquitin binding
thiol-dependent ubiquitin-specific protease activity
protein binding
omega peptidase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
cysteine protease
response to ischemia
ubiquitin-dependent protein catabolic process
axon target recognition
adult walking behavior
cell proliferation
axonal transport of mitochondrion
cellular response to drug
eating behavior
negative regulation of MAP kinase activity
muscle fiber development
neuromuscular process
regulation of macroautophagy
protein deubiquitination
sensory perception of pain
proteasome-mediated ubiquitin-dependent protein catabolic process
proteolysis
axonogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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