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Product Details
18325-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PHD3 Fusion Protein Ag13197 (1-239 aa encoded by BC010992)
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MPLGHIMRL DLEKIALEYI VPCLHEVGFC YLDNFLGEVV GDCVLERVKQ LHCTGALRDG QLAGPRAGVS KRHLRGDQIT WIGGNEEGCE AISFLLSLID RLVLYCGSRL GKYYVKERSK AMVACYPGNG TGYVRHVDNP NGDGRCITCI YYLNKNWDAK LHGGILRIFP EGKSFIADVE PIFDRLLFFW SDRRNPHEVQ PSYATRYAMT VWYFDAEERA EAKKKFRNLT RKTESALTED (1-239 aa encoded by BC010992)
Target Information
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Egl nine homolog 3; egl nine-like protein 3 isoform; egl-9 family hypoxia-inducible factor 3; FLJ21620; HIF prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 3; MGC125998; MGC125999; P4H3; PHD3; Prolyl hydroxylase domain-containing protein 3; Prolyl hydroxylase EGLN3
Gene Aliases: EGLN3; HIFP4H3; HIFPH3; PHD3
UniProt ID: (Human) Q9H6Z9
Entrez Gene ID: (Human) 112399
response to hypoxia
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
protein hydroxylation
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
regulation of cell proliferation
regulation of neuron apoptotic process
oxidation-reduction process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
Performance Guarantee
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