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          • Primary Antibodies ›
          • PHF1 Antibodies

          Invitrogen

          PHF1 Polyclonal Antibody

          View all (15) PHF1 antibodies

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          Cite PHF1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          PHF1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          PHF1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          PHF1 Antibody (PA5-27568) in WB

          Western Blot using PHF1 Polyclonal Antibody (Product # PA5-27568). Sample (30 µg of whole cell lysate). A: A431. B: H1299. 10% SDS PAGE. PHF1 Polyclonal Antibody (Product # PA5-27568) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          PHF1 Antibody in Western Blot (WB)

          Product Details

          PA5-27568

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human PHF1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          5.78 mg/mL

          Amount

          578 µg

          Purification

          Protein A

          Storage buffer

          0.1M tris glycine, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2545044

          Product Specific Information

          Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

          Predicted reactivity: Mouse (99%), Rat (99%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: alternative spliced isoform of PHF1; PHF2; hPCl1; PHD finger protein 1; polycomb-like 1; Polycomb-like protein 1; Protein PHF1; testicular tissue protein Li 140; tudor domain containing 19C; unnamed protein product

          View more View less

          Gene Aliases: hPHF1; MTF2L2; PCL1; PHF1; PHF2; TDRD19C

          View more View less

          UniProt ID: (Human) O43189

          View more View less

          Entrez Gene ID: (Human) 5252

          View more View less

          Function(s)
          transcription corepressor binding DNA binding chromatin binding protein binding zinc ion binding identical protein binding metal ion binding histone H3K4me3 reader activity histone H3K36me3 reader activity histone methyltransferase binding
          Process(es)
          chromatin organization cellular response to DNA damage stimulus negative regulation of gene expression, epigenetic DNA repair-dependent chromatin remodeling
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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