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Western blot analysis of PHF2 using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract (C) 30 µg HeLa whole cell extract and D) 30 µg HepG2 whole cell extract. Samples were loaded onto a 5% SDS-PAGE gel and probed with a PHF2 polyclonal antibody (Product # PA5-35949) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 431 and 680 of PHF2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reacts with the novel (plant homeodomain)/ LAP (leukemia-associated protein) PHD finger protein PHF-2. PHF-2 is widely expressed and is implicated in hereditary sensory neuropathy type 1 (HSN1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CENP-35; centromere protein 35; JHDM1E; jumonji C domain-containing histone demethylase 1E
CENP-35; GRC5; JHDM1E; KIAA0662; PHF2