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Product Details
CF806876
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI9C2 |
Immunogen |
Full length human recombinant protein of human PHGDH produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase
Gene Aliases: 3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA
UniProt ID: (Human) O43175
Entrez Gene ID: (Human) 26227
Molecular Function:
dehydrogenase
glutamine metabolic process
glycine metabolic process
L-serine biosynthetic process
threonine metabolic process
brain development
serine family amino acid biosynthetic process
gamma-aminobutyric acid metabolic process
regulation of gene expression
taurine metabolic process
spinal cord development
glial cell development
neural tube development
neuron projection development
oxidation-reduction process
G1 to G0 transition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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