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Invitrogen
PHKB Polyclonal Antibody
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FIGURE: 1 / 1
PHKB Antibody (PA5-96901) in WB
Western blot analysis of PHKB in extracts of various cell lines. Samples were incubated with PHKB Polyclonal antibody (Product # PA5-96901) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-96901
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 854-1093 of human PHKB (NP_0002841)
Conjugate
Form
Liquid
Concentration
1.31 mg/mL
Amount
131 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VIHIGWIISN NPELFSGMLK IRIGWIIHAM EYELQIRGGD KPALDLYQLS PSEVKQLLLD ILQPQQNGRC WLNRRQIDGS LNRTPTGFYD RVWQILERTP NGIIVAGKHL PQQPTLSDMT MYEMNFSLLV EDTLGNIDQP QYRQIVVELL MVVSIVLERN PELEFQDKVD LDRLVKEAFN EFQKDQSRLK EIEKQDDMTS FYNTPPLGKR GTCSYLTKAV MNLLLEGEVK PNNDDPCLIS; Positive Samples: HepG2, SW620, Jurkat, BT474; Cellular Location: Cell membrane, Cytoplasmic side, Lipid-anchor
Target Information
Phosphorylase kinase is a hexadecameric enzyme that is comprised of four copies of four subunits that are encoded by four separate genes: PHKA, PHKB, PHKG, and PHKD. This serine/threonine specific kinase converts glycogen phosphorylase b to glycogen phosphorylase a, resulting in the release of glucose-1-phophate from glycogen. PHKB (Phosphorylase b kinase regulatory subunit beta) is a 1093 amino acid subunit of phosphorylase kinase that, along with PHKA, has regulatory functions controlled by phosphorylation. Defects in the gene encoding PHKB are the cause of glycogen storage disease type 9B, which is also known as phosphorylase kinase deficiency of liver and muscle. This disease is characterized by a mild phenotype of hepatomegaly with only slightly elevated transaminase and plasma lipids, no clinical muscle involvement, and generally is correlated with a gradual improvement with increasing age. There are four isoforms of PHKB that are produced as a result of alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Phosphorylase B; Phosphorylase b kinase regulatory subunit beta; phosphorylase kinase; phosphorylase kinase beta-subunit; Phosphorylase kinase subunit beta; phosphorylase kinase, beta; unnamed protein product
Gene Aliases: AI463271; PHKB
UniProt ID: (Human) Q93100, (Mouse) Q7TSH2
Entrez Gene ID: (Human) 5257, (Mouse) 102093
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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