Proteintech

PHLPP Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PHLPP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using PHLPP antibody 22789-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PHLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver slide using 22789-1-AP ( PHLPP Antibody) at dilution of 1:50.

PHLPP Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PHLPP Antibody in Immunoprecipitation (IP) — IP result of anti-PHLPP (IP:22789-1-AP, 4ug; Detection:22789-1-AP 1:600) with mouse brain tissue lysate 4000 ug.

Product Details

22789-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PHLPP Fusion Protein Ag18794 (1068-1204 aa encoded by BC126277) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.57 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: QQHLLQVPA EASDEGIVIS ANEDEPGLPR KADFSAVGTI GRRRANGSVA PQERSHNVIE VATDAPLRKP GGYFAAPAQP DPDDQFIIPP ELEEEVKEIM KHHQEQQQQQ QPPPPPQLQP QLPRHYQLDQ LPDYYDTP (1068-1204 aa encoded by BC126277)

Target Information

PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.

Cell death discovery

Xiong X,Wen YA,Mitov MI,C Oaks M,Miyamoto S,Gao T

Wed Sep 30 00:00:00 EDT 2020

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 EDT 2017

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 EDT 2017

Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

The Journal of biological chemistry

Xiong X,Li X,Wen YA,Gao T

Fri Nov 25 00:00:00 EST 2016

Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

The Journal of biological chemistry

Xiong X,Li X,Wen YA,Gao T

Fri Nov 25 00:00:00 EST 2016

PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.

Oncotarget

Smith AJ,Wen YA,Stevens PD,Liu J,Wang C,Gao T

Tue Feb 16 00:00:00 EST 2016

Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis.

Biochimica et biophysica acta

Wen YA,Li X,Goretsky T,Weiss HL,Barrett TA,Gao T

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Circadian Oscillatory Protein (SCOP); EC 3.1.3.16; hSCOP; PH domain leucine-rich repeat protein phosphatase 1; PH domain leucine-rich repeat-containing protein phosphatase 1; PH domain-containing family E member 1; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1; Pleckstrin homology domain-containing family E member 1; pleckstrin homology domain-containing family E protein 1; protein phosphatase, Mg2+/Mn2+ dependent 3A; SCN circadian oscillatory protein; Suprachiasmatic nucleus circadian oscillatory protein

Gene Aliases: AI836256; KIAA0606; mKIAA0606; PHLPP; PHLPP1; PLEKHE1; PPM3A; SCOP

UniProt ID: (Human) O60346, (Mouse) Q8CHE4, (Rat) Q9WTR8

Entrez Gene ID: (Human) 23239, (Mouse) 98432, (Rat) 59265

Molecular Function: scaffold/adaptor protein