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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid synthetic peptide near the amino terminus of human PHLPP1|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||25 ug/ml|
|Immunofluorescence (IF)||25 ug/ml|
|Western Blot (WB)||1 - 2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is SW480 cell lysate.
PA5-34434 can be used with blocking peptide PEP-1476.
PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PH domain and leucine rich repeat protein phosphatase 1; PH domain-containing family E member 1; PHLPP; PHLPP1; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1; PLEKHE1; protein phosphatase, Mg2+/Mn2+ dependent 3A; SCN circadian oscillatory protein; SCOP; suprachiasmatic nucleus circadian oscillatory protein
KIAA0606; PHLPP; PHLPP1; PLEKHE1; PPM3A; SCOP