Immunofluorescent analysis of PHOX2B showing staining in the nucleus of SK-N-AS cells. SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PHOX2B polyclonal antibody (Product # PA5-29025) diluted at 1:1000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 12 and 107 of PHOX2B|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29025 targets PHOX2B in WB applications and shows reactivity with Human samples.
The PA5-29025 immunogen is a synthetic peptide corresponding to a region within amino acids 12 and 107 of PHOX2B.
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.
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