Proteintech

PHYH Polyclonal Antibody

PHYH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PHYH Antibody (12858-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens).

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Product Details

12858-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PHYH Fusion Protein Ag3569 (1-338 aa encoded by BC029512)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody (12858-1-AP) detects two bands: 36 kDa and 70 kDa. The 70 kDa band may probably be the dimeric form of PHYH.

Immunogen sequence: MEQLRAAAR LQIVLGHLGR PSAGAVVAHP TSGTISSASF HPQQFQYTLD NNVLTLEQRK FYEENGFLVI KNLVPDADIQ RFRNEFEKIC RKEVKPLGLT VMRDVTISKS EYAPSEKMIT KVQDFQEDKE LFRYCTLPEI LKYVECFTGP NIMAMHTMLI NKPPDSGKKT SRHPLHQDLH YFPFRPSDLI VCAWTAMEHI SRNNGCLVVL PGTHKGSLKP HDYPKWEGGV NKMFHGIQDY EENKARVHLV MEKGDTVFFH PLLIHGSGQN KTQGFRKAIS CHFASADCHY IDVKGTSQEN IEKEVVGIAH KFFGAENSVN LKDIWMFRAR LVKGERTNL (1-338 aa encoded by BC029512 )

Target Information

PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomics analysis reveals the effect of 1α,25(OH)₂VD₃-glycosides on development of early testes in piglets.

Scientific reports

Chen H,Bühler K,Zhu Y,Nie X,Liu W

Mon May 31 00:00:00 EDT 2021

Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics for the investigation of the effect of Hugan Qingzhi on non-alcoholic fatty liver disease in rats.

Journal of ethnopharmacology

Yao X,Xia F,Tang W,Xiao C,Yang M,Zhou B

Thu Feb 15 00:00:00 EST 2018

Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics for the investigation of the effect of Hugan Qingzhi on non-alcoholic fatty liver disease in rats.

Journal of ethnopharmacology

Yao X,Xia F,Tang W,Xiao C,Yang M,Zhou B

Thu Feb 15 00:00:00 EST 2018

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Oncotarget

Zeng HL,Yang Q,Du H,Li H,Shen Y,Liu T,Chen X,Kamal GM,Guan Q,Cheng L,Wang J,Xu F

Fri Nov 24 00:00:00 EST 2017

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Oncotarget

Zeng HL,Yang Q,Du H,Li H,Shen Y,Liu T,Chen X,Kamal GM,Guan Q,Cheng L,Wang J,Xu F

Fri Nov 24 00:00:00 EST 2017

Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.

PLoS genetics

Mizuno Y,Ninomiya Y,Nakachi Y,Iseki M,Iwasa H,Akita M,Tsukui T,Shimozawa N,Ito C,Toshimori K,Nishimukai M,Hara H,Maeba R,Okazaki T,Alodaib AN,Al Amoudi M,Jacob M,Alkuraya FS,Horai Y,Watanabe M,Motegi H,Wakana S,Noda T,Kurochkin IV,Mizuno Y,Schönbach C,Okazaki Y

Thu Jun 13 00:00:00 EDT 2013

Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.

PLoS genetics

Thu Jun 13 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 1600020H07Rik; HPCL; HPCL2; Lupus nephritis-associated peptide 1; OTTHUMP00000019131; OTTHUMP00000019132; OTTHUMP00000179083; OTTHUMP00000216226; PhyH; PHYH2; Phytanic acid oxidase; phytanoil-CoA alpha hydroxylase; phytanoyl-CoA 2 oxoglutarate dioxygenase; Phytanoyl-CoA alpha-hydroxylase; Phytanoyl-CoA dioxygenase, peroxisomal; phytanoyl-CoA hydroxylase (Refsum disease)

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Gene Aliases: AI256161; AI265699; LN1; LNAP1; PAHX; PHYH; PHYH1; RD

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UniProt ID: (Human) O14832, (Mouse) O35386, (Rat) P57093

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Entrez Gene ID: (Human) 5264, (Mouse) 16922, (Rat) 114209

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Function(s)

protein binding L-ascorbic acid binding metal ion binding cofactor binding phytanoyl-CoA dioxygenase activity catalytic activity oxidoreductase activity dioxygenase activity hydroxylase

Process(es)

fatty acid alpha-oxidation isoprenoid metabolic process methyl-branched fatty acid metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHYH Polyclonal Antibody

PHYH Antibody (12858-1-AP) in IHC (P)

Product Details

12858-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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