Invitrogen

PI3K p85 alpha Polyclonal Antibody

View all (30) PI3K p85 alpha antibodies

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Datasheet
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PI3K p85 alpha Polyclonal Antibody

Product Details

PA5-85354

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p85 alpha. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792496

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (91%), Rat (89%), Rhesus Monkey (97%), Bovine (93%).

Positive Control: Jurkat, Raji, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p55-GAMMA; p55PIK; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; RP4-533D7.2

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Gene Aliases: AGM7; GRB1; IMD36; p50alpha; p55alpha; p85; p85-ALPHA; p85alpha; PI3K; PIK3R1

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UniProt ID: (Human) P27986, (Mouse) P26450

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Entrez Gene ID: (Human) 5295, (Mouse) 18708

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Function(s)
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity receptor binding platelet-derived growth factor receptor binding calmodulin binding protein C-terminus binding protein kinase binding protein domain specific binding estrogen receptor binding receptor tyrosine kinase binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding kinase modulator
Process(es)
cellular glucose homeostasis negative regulation of cell-matrix adhesion protein phosphorylation phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation B cell differentiation positive regulation of cell migration T cell costimulation positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway positive regulation of transcription factor import into nucleus negative regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein stabilization T cell receptor signaling pathway leukocyte migration regulation of stress fiber assembly NFAT protein import into nucleus growth hormone receptor signaling pathway positive regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response positive regulation of glucose import in response to insulin stimulus positive regulation of protein phosphorylation glucose metabolic process transport negative regulation of cell adhesion signal transduction negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process protein transport negative regulation of cell-cell adhesion response to insulin positive regulation of myoblast differentiation negative regulation of blood pressure negative regulation of proteolysis negative regulation of smooth muscle cell proliferation response to glucocorticoid response to cAMP regulation of establishment of protein localization to plasma membrane
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