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PI4K alpha, type I Polyclonal Antibody
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FIGURE: 1 / 1
PI4K alpha, type I Antibody (600-401-J91) in IHC (P)
Immunohistochemistry of Rabbit anti-PIP5K1A antibody. Tissue: Tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIP5K1A antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIP5K1A as precipitated red signal with hematoxylin purple nuclear counterstain.
Product Details
600-401-J91
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-PI4K, type I alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein.
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with PI4K, type I alpha from other sources has not been determined.
Target Information
PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'disheveled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha; unnamed protein product
Gene Aliases: PIP5K1A
UniProt ID: (Human) Q99755
Entrez Gene ID: (Human) 8394
lipid metabolic process
glycerophospholipid metabolic process
phosphatidylinositol biosynthetic process
phagocytosis
signal transduction
phospholipid biosynthetic process
fibroblast migration
cell migration
actin cytoskeleton organization
keratinocyte differentiation
phosphatidylinositol metabolic process
phosphatidylinositol phosphorylation
focal adhesion assembly
regulation of protein kinase B signaling
cell chemotaxis
protein localization to plasma membrane
activation of GTPase activity
ruffle assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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