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Abnova

PIAS1/2/3 Recombinant Rabbit Monoclonal Antibody

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Product Details

RAB06469

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-

Immunoprecipitation (IP)

1:20
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

PIAS1, PIAS2, and PIAS3 are members of the Protein Inhibitor of Activated STAT (PIAS) family, which are integral to cellular processes such as SUMOylation, transcriptional regulation, and signal transduction. PIAS1 is noted for its role as a chromatin-bound androgen receptor (AR) coregulator, influencing AR-mediated target gene regulation in prostate cancer cells. It interacts with and modifies AR via SUMO3, which affects cell proliferation. PIAS2, another member, has been implicated in the inhibition of the JAK-STAT signaling pathway, notably suppressing STAT4-dependent gene induction linked to IL-12. Furthermore, PIAS2 has been shown to repress IFN-B production, highlighting its regulatory role in immune response mechanisms. PIAS3 is recognized as a SUMO E3 ligase that binds activated STAT3 and inhibits its DNA binding ability, subsequently hindering STAT3-mediated gene activation. Elevated expression of PIAS3 in prostate cancer samples has been associated with apoptosis induction. Together, the PIAS family genes are increasingly acknowledged in cancer biology due to their contributions to growth regulation and metastasis, while also representing potential therapeutic targets in cancer treatment strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Androgen receptor-interacting protein 3; AR interacting protein; ARIP3; DAB2-interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; DIP; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein ligase PIAS2; E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS1; E3 SUMO-protein transferase PIAS2; E3 SUMO-protein transferase PIAS3; GBP; Gu-binding protein; KChAP; Miz1; Msx-interacting zinc finger protein; Msx-interacting-zinc finger; Msx-interacting-zinc finger protein; nuclear protein that binds to nucleolar RNA helicase Gu protein and to p53; PIAS-NY protein; PIAS1/GBP; PIAS3; PIASxalpha6; PIG23; potassium channel regulatory protein KChAP; Potassium channel-associated protein; Protein inhibitor of activated STAT protein 1; Protein inhibitor of activated STAT protein 3; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2; RING-type E3 ubiquitin transferase PIAS1; RING-type E3 ubiquitin transferase PIAS2; RNA helicase II-binding protein; unnamed protein product; zinc finger, MIZ-type containing 3; zinc finger, MIZ-type containing 4; zinc finger, MIZ-type containing 5

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Gene Aliases: 2900068C24Rik; 6330408K17Rik; AI462206; ARIP3; AU018068; DDXBP1; Dib; DIP; GBP; GU/RH-II; MIZ1; PIAS1; PIAS2; PIAS3; Pias3l; PIASX; PIASxalpha; PIASxb; PIASxbeta; SIZ2; ZMIZ3; ZMIZ4; ZMIZ5

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UniProt ID: (Human) O75925, (Human) O75928, (Human) Q9Y6X2, (Mouse) O88907, (Rat) Q6AZ28, (Mouse) Q8C5D8, (Mouse) O54714, (Rat) O70260

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Entrez Gene ID: (Human) 8554, (Human) 9063, (Human) 10401, (Mouse) 56469, (Rat) 300772, (Rat) 83422, (Mouse) 17344, (Mouse) 229615, (Rat) 83614

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Function(s)
transcription regulatory region sequence-specific DNA binding DNA binding transcription cofactor activity transcription corepressor activity protein binding zinc ion binding transferase activity SUMO transferase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding metal ion binding SUMO ligase activity DNA-binding transcription factor binding RNA polymerase II sequence-specific DNA binding transcription factor binding protein C-terminus binding transcription factor binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity estrogen receptor binding glucocorticoid receptor binding androgen receptor binding potassium channel regulator activity protein N-terminus binding
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression protein sumoylation positive regulation of protein sumoylation positive regulation of membrane potential regulation of transcription from RNA polymerase II promoter spermatogenesis metabolic process response to organic substance androgen receptor signaling pathway response to testosterone negative regulation of sequence-specific DNA binding transcription factor activity regulation of osteoblast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of dendrite morphogenesis regulation of androgen receptor signaling pathway negative regulation of androgen receptor signaling pathway transforming growth factor beta receptor signaling pathway endoplasmic reticulum unfolded protein response negative regulation of transcription, DNA-templated regulation of macromolecule metabolic process SMAD protein signal transduction G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter JAK-STAT cascade positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of cell proliferation negative regulation of apoptotic process fat cell differentiation positive regulation of smooth muscle cell differentiation protein-DNA complex assembly negative regulation of nucleic acid-templated transcription double-strand break repair via homologous recombination DNA double-strand break processing cellular response to DNA damage stimulus visual learning positive regulation of protein localization to cell periphery response to hormone negative regulation of gene expression regulation of tumor necrosis factor-mediated signaling pathway negative regulation of protein sumoylation negative regulation of osteoclast differentiation
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