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PIAS1 Polyclonal Antibody
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FIGURE: 1 / 3
PIAS1 Antibody (600-401-DM6) in ICC/IF
Immunofluorescence Microscopy of PIAS1 antibody. Tissue: Rat kidney tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PIAS1 antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PIAS1 is nuclear. Staining: PIAS1 as red fluorescent signal.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-DM6
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the C-terminus of human PIAS1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AR interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein transferase PIAS1; Gu binding protein; gu-binding protein; nuclear protein that binds to nucleolar RNA helicase Gu protein and to p53; PIAS1; PIAS1/GBP; PIG23; protein inhibitor of activated STAT protein 1; RING-type E3 ubiquitin transferase PIAS1; RNA helicase II-binding protein; unnamed protein product; zinc finger, MIZ-type containing 3
Gene Aliases: 2900068C24Rik; DDXBP1; GBP; GU/RH-II; ZMIZ3
UniProt ID: (Human) O75925, (Mouse) O88907
Entrez Gene ID: (Human) 8554, (Mouse) 56469, (Rat) 300772
transcription regulatory region sequence-specific DNA binding
transcription cofactor activity
transcription corepressor activity
protein binding
zinc ion binding
SUMO transferase activity
enzyme binding
protein domain specific binding
ubiquitin protein ligase binding
SUMO ligase activity
DNA-binding transcription factor binding
transcription regulator inhibitor activity
ubiquitin protein ligase activity
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
DNA double-strand break processing
cellular response to DNA damage stimulus
JAK-STAT cascade
spermatogenesis
visual learning
protein sumoylation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of protein sumoylation
regulation of cell proliferation
negative regulation of apoptotic process
fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
positive regulation of smooth muscle cell differentiation
positive regulation of transcription initiation from RNA polymerase II promoter
protein-DNA complex assembly
positive regulation of protein localization to cell periphery
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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