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Invitrogen
PIAS3 Polyclonal Antibody
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FIGURE: 1 / 6
PIAS3 Antibody (PA5-104467) in ICC/IF
Immunocytochemistry analysis of PIAS3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PIAS3 polyclonal antibody (Product # PA5-104467) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104467
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIAS3(Accession Q9Y6X2), corresponding to amino acid residues G5-M55.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PIAS3.
Target Information
The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS3; KChAP; PIAS3; potassium channel regulatory protein KChAP; Potassium channel-associated protein; Protein inhibitor of activated STAT protein 3; unnamed protein product; zinc finger, MIZ-type containing 5
Gene Aliases: PIAS3; Pias3l; ZMIZ5
UniProt ID: (Human) Q9Y6X2, (Mouse) O54714, (Rat) O70260
Entrez Gene ID: (Human) 10401, (Mouse) 229615, (Rat) 83614
transcription cofactor activity
protein binding
zinc ion binding
transferase activity
SUMO transferase activity
metal ion binding
SUMO ligase activity
protein C-terminus binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
potassium channel regulator activity
ligase activity
ribosomal S6-glutamic acid ligase activity
enzyme binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein N-terminus binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
protein sumoylation
positive regulation of protein sumoylation
positive regulation of membrane potential
response to hormone
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of protein sumoylation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
regulation of macromolecule metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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