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Invitrogen
PIASy Polyclonal Antibody
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FIGURE: 1 / 23
PIASy Antibody (PA5-99455) in IHC (P)
Immunohistochemistry analysis of PIASy in mouse colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIASy polyclonal antibody (Product # PA5-99455) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99455
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIAS4(Accession Q8N2W9), corresponding to amino acid residues L468-C510.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PIAS4.
Target Information
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: e3 sumo- ligase pias4; FLJ12419; MGC35296; polymorphic variant; PIASy; protein inhibitor of activated STAT PIASy; protein inhibitor of activated STAT protein 4; protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; RING-type E3 ubiquitin transferase PIAS4; unnamed protein product; zinc finger, MIZ-type containing 6
Gene Aliases: PIAS-gamma; Piasg; PIASY; ZMIZ6
UniProt ID: (Human) Q8N2W9, (Mouse) Q9JM05
Entrez Gene ID: (Human) 51588, (Mouse) 59004, (Rat) 362827
negative regulation of transcription from RNA polymerase II promoter
hair follicle development
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
JAK-STAT cascade
central nervous system development
regulation of gene expression
negative regulation of tumor necrosis factor-mediated signaling pathway
protein sumoylation
positive regulation of protein sumoylation
negative regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
negative regulation of transcription, DNA-templated
negative regulation of JAK-STAT cascade
limb epidermis development
mRNA destabilization
regulation of cellular response to stress
negative regulation of protein localization to chromatin
positive regulation of keratinocyte apoptotic process
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of double-strand break repair via homologous recombination
negative regulation of double-strand break repair via homologous recombination
biological_process
double-strand break repair
vitamin D metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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