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Invitrogen

PIASy Polyclonal Antibody

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PIASy Polyclonal Antibody

Product Details

PA5-99455

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PIAS4(Accession Q8N2W9), corresponding to amino acid residues L468-C510.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818388

Product Specific Information

Antibody detects endogenous levels of total PIAS4.

Target Information

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; polymorphic variant; PIASy; protein inhibitor of activated STAT PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; RING-type E3 ubiquitin transferase PIAS4; unnamed protein product; zinc finger, MIZ-type containing 6

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Gene Aliases: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6

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UniProt ID: (Human) Q8N2W9, (Mouse) Q9JM05

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Entrez Gene ID: (Human) 51588, (Rat) 362827, (Mouse) 59004

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Function(s)
DNA binding transcription cofactor activity transcription corepressor activity protein binding zinc ion binding transferase activity SUMO transferase activity ubiquitin protein ligase binding metal ion binding ubiquitin protein ligase activity SUMO ligase activity protein C-terminus binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter hair follicle development double-strand break repair regulation of transcription from RNA polymerase II promoter cellular response to DNA damage stimulus central nervous system development regulation of gene expression negative regulation of macromolecule biosynthetic process negative regulation of tumor necrosis factor-mediated signaling pathway Wnt signaling pathway protein sumoylation positive regulation of protein sumoylation vitamin D metabolic process negative regulation of I-kappaB kinase/NF-kappaB signaling regulation of mRNA stability negative regulation of transcription, DNA-templated limb epidermis development mRNA destabilization regulation of cellular response to stress negative regulation of protein localization to chromatin positive regulation of keratinocyte apoptotic process positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of double-strand break repair via homologous recombination negative regulation of double-strand break repair via homologous recombination biological_process metabolic process negative regulation of NF-kappaB transcription factor activity transcription, DNA-templated regulation of transcription, DNA-templated JAK-STAT cascade multicellular organism development viral process negative regulation of nucleic acid-templated transcription
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