Bethyl Laboratories

PIBF Polyclonal Antibody

View all (12) PIBF1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PIBF Antibody in Western Blot (WB) — **Detection of human PIBF1 by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-PIBF1 antibody A301-156A used for WB at 0.04 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. PIBF1 was also immunoprecipitated by rabbit anti-PIBF1 antibody A301-155A, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Product Details

A301-156A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 735 and 757

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Centrosomal protein of 90 kDa; CEP90; PIBF; progesterone-induced blocking factor 1; Progesterone-induced-blocking factor 1; RP11-505F3.1

Gene Aliases: C13orf24; CEP90; PIBF; PIBF1

UniProt ID: (Human) Q8WXW3

Entrez Gene ID: (Human) 10464

Function(s)

immune system process mitotic metaphase plate congression activation of JAK1 kinase activity negative regulation of prostaglandin biosynthetic process negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of natural killer cell activation nonmotile primary cilium assembly negative regulation of tyrosine phosphorylation of Stat4 protein positive regulation of tyrosine phosphorylation of Stat6 protein protein localization to centrosome mitotic spindle assembly

Process(es)

interleukin-4 receptor binding protein binding