Bethyl Laboratories

PIBF Polyclonal Antibody

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PIBF Antibody in Western Blot (WB) — **Detection of human PIBF1 by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-PIBF1 antibody A301-156A used for WB at 0.04 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. PIBF1 was also immunoprecipitated by rabbit anti-PIBF1 antibody A301-155A, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Product Details

A301-156A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 735 and 757 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.20 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Centrosomal protein of 90 kDa; CEP90; PIBF; progesterone-induced blocking factor 1; Progesterone-induced-blocking factor 1; RP11-505F3.1

Gene Aliases: C13orf24; CEP90; PIBF; PIBF1

UniProt ID: (Human) Q8WXW3

Entrez Gene ID: (Human) 10464

Function(s)