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Alomone Labs, LTD
PICK1 Polyclonal Antibody
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FIGURE: 1 / 3
PICK1 Antibody (APZ-014-200UL) in IHC (F)
Expression of PICK1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 Antibody (#APZ-014), (1:400), followed by Anti-rabbit-Cy3 Antibody . PICK1 staining (red in the hippocampal CA3 region) is detected in neurons (arrows). Nuclei are stained with DAPI (blue).
Product Details
APZ-014-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1, Intracellular, alpha-helix domain
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Amount
160 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC15204; OTTHUMP00000028509; PRKCA-binding protein; PRKCABP; Protein interacting with C kinase 1; protein kinase C, alpha binding protein; Protein kinase C-alpha-binding protein; protein that interacts with C kinase 1; unnamed protein product
Gene Aliases: PICK; PICK1; PRKCABP
UniProt ID: (Human) Q9NRD5, (Rat) Q9EP80
Entrez Gene ID: (Human) 9463, (Mouse) 18693, (Rat) 84591
G-protein coupled receptor binding
actin binding
protein kinase C binding
receptor binding
protein binding
phospholipid binding
protein domain specific binding
identical protein binding
metal ion binding
actin filament binding
Arp2/3 complex binding
membrane curvature sensor activity
protein C-terminus binding
ATPase activity
enzyme binding
kinase activity
receptor tyrosine kinase binding
G-protein coupled glutamate receptor binding
ephrin receptor binding
GTPase binding
vesicle coat protein
positive regulation of receptor internalization
protein phosphorylation
intracellular protein transport
monoamine transport
glial cell development
regulation of Arp2/3 complex-mediated actin nucleation
negative regulation of Arp2/3 complex-mediated actin nucleation
cellular response to decreased oxygen levels
cellular response to glucose starvation
receptor clustering
neuronal ion channel clustering
regulation of insulin secretion
long term synaptic depression
dendritic spine organization
dendritic spine maintenance
protein targeting
protein kinase C-activating G-protein coupled receptor signaling pathway
multicellular organism development
negative regulation of gene expression
intracellular signal transduction
regulation of synaptic plasticity
regulation of synapse structure or activity
protein homooligomerization
negative regulation of cell death
dopamine transport
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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