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Invitrogen
PICK1 Polyclonal Antibody
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Product Details
PA1-073
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-073 detects protein interacting with protein kinase C (PICK1) from mouse cells.
PA1-073 has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~52 kDa protein representing PICK1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
The PA1-073 immunogen is a synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1. The PA1-073 immunizing peptide (Cat. # PEP-133) is available for use in neutralization and control experiments.
Target Information
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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