|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Non-human primate, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1.7 µg/ml|
|Immunofluorescence (IF)||1-2 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-073 detects protein interacting with protein kinase C (PICK1) from mouse cells.
PA1-073 has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~52 kDa protein representing PICK1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
The PA1-073 immunogen is a synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1. The PA1-073 immunizing peptide (Cat. # PEP-133) is available for use in neutralization and control experiments.
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EphrinBs regulate D-serine synthesis and release in astrocytes.
PA1-073 was used in western blot to investigate the role of EphrinBs in D-serine synthesis and release in astrocytes
|Zhuang Z,Yang B,Theus MH,Sick JT,Bethea JR,Sick TJ,Liebl DJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:16015)||2010|
Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-alpha.
PA1-073 was used in western blot to investigate the interaction between PICK1 and PKC alpha
|Staudinger J,Lu J,Olson EN||The Journal of biological chemistry (272:32019)||1997|
|Non-human primate||1.7 ug/ml||
Surface expression of the netrin receptor UNC5H1 is regulated through a protein kinase C-interacting protein/protein kinase-dependent mechanism.
PA1-073 was used in immunocytochemistry to investigate the expression of the netrin receptor UNC5H1.
|Williams ME,Wu SC,McKenna WL,Hinck L||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:11279)||2003|