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Invitrogen
PIF1 Polyclonal Antibody
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Product Details
PA5-37136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to residues in human PIF1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 70 kDa.
Purity is >95% by SDS-PAGE.
Target Information
PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent DNA helicase PIF1; ATP-dependent DNA helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}; DNA helicase-like protein; DNA repair and recombination helicase PIF1; DNA repair and recombination helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}; petite integration frequency 1; PIF1 5'-to-3' DNA helicase homolog; PIF1 homolog; PIF1/RRM3 DNA helicase-like protein; Pif1/Rrm3 DNA-helicase-like protein
Gene Aliases: 4631410M14; AI449441; C15orf20; PIF; PIF1
UniProt ID: (Human) Q9H611, (Rat) Q1HG60, (Mouse) Q80SX8
Entrez Gene ID: (Human) 80119, (Rat) 367645, (Mouse) 208084
magnesium ion binding
ATP binding
telomerase inhibitor activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
ATP-dependent 5'-3' DNA/RNA helicase activity
telomeric DNA binding
ATP-dependent 5'-3' DNA helicase activity
G-quadruplex DNA binding
nucleotide binding
DNA binding
DNA helicase activity
helicase activity
hydrolase activity
DNA helicase
mitochondrial genome maintenance
telomere maintenance
DNA repair
DNA recombination
regulation of telomere maintenance
negative regulation of telomere maintenance via telomerase
DNA duplex unwinding
G-quadruplex DNA unwinding
negative regulation of telomerase activity
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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