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Agrisera
PIF4/Phytochrome interacting factor 4 Polyclonal Antibody
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FIGURE: 1 / 1
PIF4/Phytochrome interacting factor 4 Antibody (AS16-3955) in WB
30 µL of total protein from Arabidopsis thaliana grown in 12 hour light/ 12 hour dark growth conditions and 6-day-old seedling samples taken at the end of the day (ZT 12) were extracted with 100 m M MOPS, p H 7.6,100 m M Na Cl, 10% glycerol, 40 m M 2-mercaptoethanol, 5% SDS, 1 X protease inhibitor cocktail from Roche, 2 m M PMSF. 80 µL buffer were added to about 100 µL grinded powder, then immediately heate... View More
Product Details
AS16-3955
Product Specifications
Species Reactivity
Plant
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana PIF4.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
Transcription factor acting negatively in the phytochrome B signaling pathway. May regulate the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Activated by CRY1 and CRY2 in response to low blue light (LBL) by direct binding at chromatin on E-box variant 5'-CA[CT]GTG-3' to stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation in low blue light). [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtPIF4; BHLH9; MFL8.13; MFL8_13; SRL2
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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