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          • Primary Antibodies ›
          • PIGM Antibodies

          Invitrogen

          PIGM Polyclonal Antibody

          View all (3) PIGM antibodies

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          Cite PIGM Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          PIGM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PIGM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          PIGM Antibody (PA5-120585) in ICC/IF

          Immunofluorescence analysis of PIGM in L929 cells. Samples were incubated with PIGM Polyclonal antibody (Product # PA5-120585) using a dilution of 1:100. Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PIGM Antibody in Immunocytochemistry (ICC/IF)
          PIGM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PIGM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PIGM Antibody in Western Blot (WB)
          PIGM Polyclonal Antibody

          Product Details

          PA5-120585

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 35-96 of human PIGM (NP_660150.1).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.66 mg/mL

          Amount

          66 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2914157

          Product Specific Information

          Positive test controls include: BxPC-3, Mouse thymus, Mouse spleen, Mouse lung, Rat thymus, Rat spleen.

          Immunogen sequence: YGVFQDRTLH VRYTDIDYQV FTDAARFVTE GRSPYLRATY RYTPLLGWLL TPNIYLSELF GK

          Target Information

          PIGM encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. PIGM encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: dol-P-Man dependent GPI mannosyltransferase; DPM:GlcN-(acyl-)PI mannosyltransferase; GPI alpha-1,4-mannosyltransferase I, catalytic subunit; GPI mannosyltransferase 1; GPI mannosyltransferase I; GPI-MT-I; phosphatidylinositol glycan, class M; Phosphatidylinositol-glycan biosynthesis class M protein; PIG-M; PIG-M mannosyltransferase; PIG-M mRNA for mannosyltransferase; unnamed protein product

          View more View less

          Gene Aliases: 4933437L05Rik; AI644422; AV302080; C920011G20Rik; GPI-MT-I; PIGM

          View more View less

          UniProt ID: (Human) Q9H3S5, (Mouse) Q8C2R7, (Rat) Q9EQY6

          View more View less

          Entrez Gene ID: (Human) 93183, (Mouse) 67556, (Rat) 79112

          View more View less

          Function(s)
          mannosyltransferase activity glycolipid mannosyltransferase activity protein binding transferase activity transferase activity, transferring glycosyl groups alpha-1,4-mannosyltransferase activity dol-P-Man:GlcN-acyl-PI alpha-1,4-mannosyltransferase activity alpha-1,6-mannosyltransferase activity alpha-1,2-mannosyltransferase activity alpha-1,3-mannosyltransferase activity alpha-1,3-galactosyltransferase activity UDP-glucose:glycoprotein glucosyltransferase activity oligosaccharyl transferase activity dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity acetylglucosaminyltransferase activity acetylgalactosaminyltransferase activity galactosyltransferase activity fucosyltransferase activity O antigen polymerase activity lipopolysaccharide-1,6-galactosyltransferase activity transferase activity, transferring hexosyl groups cellulose synthase activity 9-phenanthrol UDP-glucuronosyltransferase activity 1-phenanthrol glycosyltransferase activity 9-phenanthrol glycosyltransferase activity 1,2-dihydroxy-phenanthrene glycosyltransferase activity phenanthrol glycosyltransferase activity beta-1,4-mannosyltransferase activity alpha-1,2-galactosyltransferase activity dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity lipopolysaccharide-1,5-galactosyltransferase activity dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity inositol phosphoceramide synthase activity glucosyltransferase activity alpha-(1->3)-fucosyltransferase activity alpha-(1->6)-fucosyltransferase activity indole-3-butyrate beta-glucosyltransferase activity salicylic acid glucosyltransferase (ester-forming) activity salicylic acid glucosyltransferase (glucoside-forming) activity benzoic acid glucosyltransferase activity chondroitin hydrolase activity dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity cytokinin 9-beta-glucosyltransferase activity glycosyltransferase
          Process(es)
          GPI anchor biosynthetic process mannosylation
          It has to be done as per old AB suggested Products section.

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