Proteintech

PIGZ Polyclonal Antibody

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Protocols

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PIGZ Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 17384-1-AP (PIGZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PIGZ Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 40x lens).

PIGZ Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

17384-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIGZ Fusion Protein Ag11259 (435-579 aa encoded by BC044640) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LVPGLEYLE QVVHAPVLPS TPTHYTLLFT HTYMPPRHLL HLPGLGAPVE VVDIGGTEDW ALCQTLKSFT RQPACQVAGG PWLCRLFVVT PGTTRRAVEK CSFPFKNETL LFPHLTLEDP PALSSLLSGA WRDHLSLHIV ELGEET (435-579 aa encoded by BC044640)

Target Information

The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dol-P-Man dependent GPI mannosyltransferase; GPI mannosyltransferase 4; GPI mannosyltransferase IV; GPI-MT-IV; hSMP3; phosphatidylinositol glycan anchor biosynthesis, class Z; phosphatidylinositol glycan, class Z; phosphatidylinositol glycan, class Z-like 1; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; SMP3 mannosyltransferase

Gene Aliases: F630022B06Rik; GPI-MT-IV; PIG-Z; PIGZ; RGD1307107; SMP3

UniProt ID: (Human) Q86VD9, (Mouse) Q8BTP0

Entrez Gene ID: (Human) 80235, (Mouse) 239827, (Rat) 303872

Molecular Function: glycosyltransferase transferase metabolite interconversion enzyme