Proteintech

PIH1D1 Polyclonal Antibody

View all (7) PIH1D1 antibodies

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PIH1D1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PIH1D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

PIH1D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

PIH1D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

PIH1D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

PIH1D1 Antibody in Western Blot (WB) — Human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Western Blot (WB) — Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Western Blot (WB) — Mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIH1D1 Antibody in Immunoprecipitation (IP) — IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug.

Product Details

19427-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIH1D1 Fusion Protein Ag13759 (1-290 aa encoded by BC001108) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.35 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MANPKLLGL ELSEAEAIGA DSARFEELLL QASKELQQAQ TTRPESTQIQ PQPGFCIKTN SSEGKVFINI CHSPSIPPPA DVTEEELLQM LEEDQAGFRI PMSLGEPHAE LDAKGQGCTA YDVAVNSDFY RRMQNSDFLR ELVITIAREG LEDKYNLQLN PEWRMMKNRP FMGSISQQNI RSEQRPRIQE LGDLYTPAPG RAESGPEKPH LNLWLEAPDL LLAEIDLPKL DGALGLSLEI GENRLVMGGP QQLYHLDAYI PLQINSHESK AAFHRKRKQL MVAMPLLLVP S (1-290 aa encoded by BC001108)

Target Information

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed:17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed:22368283, PubMed:24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed:20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed:24036451).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.

The Journal of biological chemistry

Vangala JR,Radhakrishnan SK

Fri Feb 08 00:00:00 EST 2019

The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.

Chromosoma

Imai Y,Baudat F,Taillepierre M,Stanzione M,Toth A,de Massy B

Fri Dec 01 00:00:00 EST 2017

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

RNA (New York, N.Y.)

Machado-Pinilla R,Liger D,Leulliot N,Meier UT

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Nucleolar protein 17 homolog; PIH1 domain-containing protein 1

Gene Aliases: 1110061L23Rik; 4933413A04Rik; NOP17; PIH1D1; RGD1309809

UniProt ID: (Human) Q9NWS0, (Mouse) Q9CQJ2, (Rat) Q4V7F5

Entrez Gene ID: (Human) 55011, (Mouse) 68845, (Rat) 292898

box C/D snoRNP assembly epithelial cell differentiation chromatin remodeling transcription, DNA-templated positive regulation of protein complex assembly snoRNA localization regulation of histone H3-K4 methylation establishment of protein localization to chromatin positive regulation of protein serine/threonine kinase activity positive regulation of histone H4 acetylation negative regulation of histone H3-K9 dimethylation negative regulation of histone H3-K9 trimethylation positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of glucose mediated signaling pathway regulation of TORC2 signaling positive regulation of TORC1 signaling positive regulation of histone H3-K9 acetylation negative regulation of histone H4-K16 acetylation negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway

Process(es)

RNA polymerase I CORE element sequence-specific DNA binding RNA polymerase I upstream control element sequence-specific DNA binding protein binding protein kinase binding histone binding ATPase binding phosphoprotein binding molecular_function