Proteintech

PIH1D1 Polyclonal Antibody

PIH1D1 Antibody in Immunocytochemistry (ICC/IF)

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PIH1D1 Antibody (19427-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PIH1D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIH1D1 Antibody in Immunoprecipitation (IP)

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Product Details

19427-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIH1D1 Fusion Protein Ag13759 (1-290 aa encoded by BC001108)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MANPKLLGL ELSEAEAIGA DSARFEELLL QASKELQQAQ TTRPESTQIQ PQPGFCIKTN SSEGKVFINI CHSPSIPPPA DVTEEELLQM LEEDQAGFRI PMSLGEPHAE LDAKGQGCTA YDVAVNSDFY RRMQNSDFLR ELVITIAREG LEDKYNLQLN PEWRMMKNRP FMGSISQQNI RSEQRPRIQE LGDLYTPAPG RAESGPEKPH LNLWLEAPDL LLAEIDLPKL DGALGLSLEI GENRLVMGGP QQLYHLDAYI PLQINSHESK AAFHRKRKQL MVAMPLLLVP S (1-290 aa encoded by BC001108)

Target Information

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed:17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed:22368283, PubMed:24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed:20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed:24036451).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.

Nucleic acids research

Abel Y,Charron C,Virciglio C,Bourguignon-Igel V,Quinternet M,Chagot ME,Robert MC,Verheggen C,Branlant C,Bertrand E,Manival X,Charpentier B,Rederstorff M

Mon Feb 28 00:00:00 UTC 2022

The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.

Nucleic acids research

Abel Y,Charron C,Virciglio C,Bourguignon-Igel V,Quinternet M,Chagot ME,Robert MC,Verheggen C,Branlant C,Bertrand E,Manival X,Charpentier B,Rederstorff M

Mon Feb 28 00:00:00 UTC 2022

The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.

Nature communications

Maurizy C,Abeza C,Lemmers B,Gabola M,Longobardi C,Pinet V,Ferrand M,Paul C,Bremond J,Langa F,Gerbe F,Jay P,Verheggen C,Tinari N,Helmlinger D,Lattanzio R,Bertrand E,Hahne M,Pradet-Balade B

Tue Aug 10 00:00:00 UTC 2021

The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.

Nature communications

Maurizy C,Abeza C,Lemmers B,Gabola M,Longobardi C,Pinet V,Ferrand M,Paul C,Bremond J,Langa F,Gerbe F,Jay P,Verheggen C,Tinari N,Helmlinger D,Lattanzio R,Bertrand E,Hahne M,Pradet-Balade B

Tue Aug 10 00:00:00 UTC 2021

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.

Cell chemical biology

Yenerall P,Das AK,Wang S,Kollipara RK,Li LS,Villalobos P,Flaming J,Lin YF,Huffman K,Timmons BC,Gilbreath C,Sonavane R,Kinch LN,Rodriguez-Canales J,Moran C,Behrens C,Hirasawa M,Takata T,Murakami R,Iwanaga K,Chen BPC,Grishin NV,Raj GV,Wistuba II,Minna JD,Kittler R

Thu Jan 16 00:00:00 UTC 2020

Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.

The Journal of biological chemistry

Vangala JR,Radhakrishnan SK

Fri Feb 08 00:00:00 UTC 2019

Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.

The Journal of biological chemistry

Vangala JR,Radhakrishnan SK

Fri Feb 08 00:00:00 UTC 2019

The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.

Chromosoma

Imai Y,Baudat F,Taillepierre M,Stanzione M,Toth A,de Massy B

Fri Dec 01 00:00:00 UTC 2017

The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.

Chromosoma

Imai Y,Baudat F,Taillepierre M,Stanzione M,Toth A,de Massy B

Fri Dec 01 00:00:00 UTC 2017

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

RNA (New York, N.Y.)

Machado-Pinilla R,Liger D,Leulliot N,Meier UT

Mon Oct 01 00:00:00 UTC 2012

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

RNA (New York, N.Y.)

Machado-Pinilla R,Liger D,Leulliot N,Meier UT

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Nucleolar protein 17 homolog; PIH1 domain-containing protein 1

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Gene Aliases: 1110061L23Rik; 4933413A04Rik; NOP17; PIH1D1; RGD1309809

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UniProt ID: (Human) Q9NWS0, (Mouse) Q9CQJ2, (Rat) Q4V7F5

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Entrez Gene ID: (Human) 55011, (Mouse) 68845, (Rat) 292898

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Function(s)

RNA polymerase I CORE element sequence-specific DNA binding RNA polymerase I upstream control element sequence-specific DNA binding protein binding protein kinase binding histone binding ATPase binding phosphoprotein binding molecular_function

Process(es)

box C/D snoRNP assembly epithelial cell differentiation chromatin remodeling transcription, DNA-templated positive regulation of protein complex assembly snoRNA localization regulation of histone H3-K4 methylation establishment of protein localization to chromatin positive regulation of protein serine/threonine kinase activity positive regulation of histone H4 acetylation negative regulation of histone H3-K9 dimethylation negative regulation of histone H3-K9 trimethylation positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of glucose mediated signaling pathway regulation of TORC2 signaling positive regulation of TORC1 signaling positive regulation of histone H3-K9 acetylation negative regulation of histone H4-K16 acetylation negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PIH1D1 Polyclonal Antibody

PIH1D1 Antibody (19427-1-AP) in ICC/IF

Product Details

19427-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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