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Product Details
CF801692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3H2
Immunogen
Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, the Class II enyzmes have been linked to diverse, receptor-mediated cellular processes including insulin signaling, neuronal survival, and growth factor signaling. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C2-containing phosphatidylinositol kinase; DKFZp686L193; MGC142218; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha; Phosphoinositide 3-kinase-C2-alpha; phosphoinositide-3-kinase, class 2, alpha polypeptide; PI3K-C2-alpha; pi3kc2a; ptdIns-3-kinase C2 subunit alpha
Gene Aliases: CPK; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha; PIK3C2A
UniProt ID: (Human) O00443
Entrez Gene ID: (Human) 5286
phosphatidylinositol biosynthetic process
exocytosis
endocytosis
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
vascular smooth muscle contraction
phosphatidylinositol-3-phosphate biosynthetic process
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
clathrin coat assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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