Invitrogen

PIK3CB Polyclonal Antibody

Product Image Gallery

PIK3CB Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human pancreas tissue using a PIK3CB polyclonal antibody (Product # PA5-15249), followed by HRP-conjugated secondary antibody and DAB staining.

PIK3CB Antibody in Western Blot (WB) — Western Blot was performed using Anti-PIK3CB Polyclonal Antibody (Product # PA5-15249) and a 123 kDa band corresponding to Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform was observed across tissues tested. Tissue extracts (30 µg lysate) of Mouse Thyroid (Lane 1), Mouse Stomach (Lane 2), Mouse Mammary Gland (Lane 3), Rat Mammary Gland (Lane 4), Mouse Ovary (Lane 5), Mouse Lung (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 60-80 kDa.

Product Details

PA5-15249

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 124-154 amino acids from the N-terminal region of human PIK3CB View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Lot-specific
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2165396

Target Information

PIK3CB (phosphatyidylinositol 4,5-biphosphate 3-kinase catalyic subunit beta isoform) is a phosphoinositide-3-kinase that phosphorylates PtdIns (phosphatidylinositol), PtdIns4P (phosphatidylinositol 4-phosphate), and PtdIns(4,5)P2 (phosphatidylinositol 4,5-biphosphate) to generate phosphatidylinositol 3,4,5-triphosphate (PIP3). PIP3 plays a key role in recruiting PH domain-containing proteins to the membrane and activating signaling cascades involved in cell growth, survival, proliferation, motility, and morphology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta; phosphoinositide-3-kinase, catalytic, beta polypeptide; PI3-kinase p110 subunit beta; PI3-kinase subunit beta; PI3K-beta; pi3kcb; PtdIns-3-kinase p110; ptdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta

Gene Aliases: P110BETA; PI3K; PI3KBETA; PIK3C1; PIK3CB

UniProt ID: (Human) P42338

Entrez Gene ID: (Human) 5291

Molecular Function: kinase