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Invitrogen
PIM1 Polyclonal Antibody
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FIGURE: 1 / 2
PIM1 Antibody (PA5-89681) in WB
Western blot analysis of PIM1 in extracts of various cell lines. Samples were incubated with PIM1 Polyclonal antibody (Product # PA5-89681) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PIM1 (NP_0012301151)
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Amount
70 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: H460, Mouse kidney, Rat kidney; Cellular Location: Cell membrane, Cytoplasm, Nucleus
Immunogen sequence: MPHEPHEPLT PPFSALPDPA GAPSRRQSRQ RPQLSSDSPS AFRASRSHSR NATRSHSHSH SPRHSLRHSP GSGSCGSSSG HRPCADILEV GMLLSKINSL AHLRAAPCND LHATKLAPGK EKEPLESQYQ VGPLLGSGGF GSVYSGIRVS DNLPVAIKHV EKDRISDWGE LPNGTRVPME VVLLKKVSSG FSGVIRLLDW FERPDSFVLI LERPEPVQDL FDFITERGAL QEELARSFFW QVLEAVRHCH NCGVLHRDIK DENILIDLNR GELKLIDFGS GALLKDTVYT DFDGTRVYSP PEWIRYHRYH GRS
Target Information
The proto-oncogene PIM1 is upregulated in prostate cancer. PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: non-specific serine/threonine protein kinase; Oncogene PIM1; pim-1 oncogene; pim-1 oncogene (proviral integration site 1); Pim-1L; proto-oncogene serine/threonine-protein kinase Pim-1; proviral integration site 1; Serine/threonine-protein kinase pim-1; unnamed protein product
Gene Aliases: PIM; Pim-1; PIM1
UniProt ID: (Human) P11309, (Rat) P26794, (Mouse) F6XDQ5
Entrez Gene ID: (Human) 5292, (Rat) 24649, (Mouse) 18712
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
transcription factor binding
kinase activity
transferase activity
manganese ion binding
ribosomal small subunit binding
protein serine/threonine kinase activator activity
metal ion binding
protein serine kinase activity
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
regulation of mitotic cell cycle
regulation of transmembrane transporter activity
cellular response to nutrient levels
cellular response to amino acid starvation
defense response to bacterium
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of innate immune response
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
cytolysis in other organism
positive regulation of cardiac muscle cell proliferation
vitamin D receptor signaling pathway
cellular response to interferon-gamma
positive regulation of protein serine/threonine kinase activity
positive regulation of brown fat cell differentiation
non-canonical inflammasome complex assembly
regulation of hematopoietic stem cell proliferation
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of cardioblast proliferation
cellular detoxification
cell proliferation
hyaluronan metabolic process
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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