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PIM2 Antibody (PA5-142734) in WB
Western Blot analysis of PIM2 in HEK293 lysate (10 µg protein in RIPA buffer) using PIM2 Polyclonal Antibody (Product # PA5-142734) at 0.1 µg/mL. Lane A: HEK293 lysate over expressing Human PIM2 with C-terminal MYC tag probed with PIM2 Antibody. Lane B: Mock-transfected HEK293 probed with PIM2 Antibody. Lane C: HEK293 lysate over expressing Human PIM2 with DYKDDDDK tag probed with anti-MYC Tag (1:1,000). Primary incubations were for 1 hour. Detected by chemiluminescence.
Product Details
PA5-142734
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-KDREAFEAEYRLGP.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933380
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000.
Target Information
PIM2 is a proto-oncogene which phosphorylates BAD, which partially inhibits its apopotopic function. PIM proteins (PIM-1, PIM-2 and PIM-3) are oncogene-encoded serine/threonine kinases. PIM-2 is highly homologous to Pim-1 with similar oncogenic functions. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. PIM2 expression is negatively regulated by growth factor depletion. Increased levels of PIM-2 has also been observed in certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pim-2 oncogene; Pim-2h; proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2; Serine/threonine-protein kinase pim-2
Gene Aliases: PIM2
UniProt ID: (Human) Q9P1W9
Entrez Gene ID: (Human) 11040
G1/S transition of mitotic cell cycle
protein phosphorylation
apoptotic process
male meiosis
regulation of mitotic cell cycle
cell proliferation
negative regulation of cell proliferation
response to virus
positive regulation of autophagy
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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