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PINX1 Polyclonal Antibody

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PINX1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PINX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin tissue slide using 12368-1-AP ( PINX1 Antibody) at dilution of 1:50 (under 40x lens).

PINX1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

12368-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PINX1 Fusion Protein Ag3043 (1-328 aa encoded by BC015479) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSMLAERRR KQKWAVDPQN TAWSNDDSKF GQRMLEKMGW SKGKGLGAQE HGATDHIKVQ VKNNHLGLGA TINNEDNWIA HQDDFNQLLA ELNTCHGQET TDSSDKKEKK SFSLEEKSKI SKNRVHYMKF TKGKDLSSRS KTDLDCIFGK RQSKKTPEGD ASPSTPEENE TTTTSAFTIQ EYFAKRMAAL KNKPQVPVPG SDISETQVER KRGKKINKEA TGKDVESYLQ PKAKRHTEGK PERAEAQERV AKKKSAPAEE QLRGPCWDQS SKASAQDAGD HVQPPEGRDF TLKPKKRRGK KKLQKPVEIA EDATLEETLV KKKKKKDSK (1-328 aa encoded by BC015479 )

Target Information

Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The clinical significance of PINX1 expression in papillary thyroid carcinoma.

Human pathology

Kang J,Han K,Kim HJ,Park JH,Kong JS,Park S,Myung JK

Thu Nov 01 00:00:00 EDT 2018

The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.

Molecular cancer

Tian XP,Jin XH,Li M,Huang WJ,Xie D,Zhang JX

Tue Apr 04 00:00:00 EDT 2017

PinX1 suppresses tumorigenesis by negatively regulating telomerase/telomeres in colorectal carcinoma cells and is a promising molecular marker for patient prognosis.

OncoTargets and therapy

Qian D,Cheng J,Ding X,Chen X,Chen X,Guan Y,Zhang B,Wang J,Er P,Qiu M,Zeng X,Guo Y,Wang H,Zhao L,Xie D,Yuan Z,Wang P,Pang Q

Thu Aug 18 00:00:00 EDT 2016

PinX1 suppresses tumorigenesis by negatively regulating telomerase/telomeres in colorectal carcinoma cells and is a promising molecular marker for patient prognosis.

OncoTargets and therapy

Qian D,Cheng J,Ding X,Chen X,Chen X,Guan Y,Zhang B,Wang J,Er P,Qiu M,Zeng X,Guo Y,Wang H,Zhao L,Xie D,Yuan Z,Wang P,Pang Q

Thu Aug 18 00:00:00 EDT 2016

The telomere/telomerase binding factor PinX1 regulates paclitaxel sensitivity depending on spindle assembly checkpoint in human cervical squamous cell carcinomas.

Cancer letters

Tian XP,Qian D,He LR,Huang H,Mai SJ,Li CP,Huang XX,Cai MY,Liao YJ,Kung HF,Zeng YX,Xie D

Fri Oct 10 00:00:00 EDT 2014

The telomere/telomerase binding factor PinX1 regulates paclitaxel sensitivity depending on spindle assembly checkpoint in human cervical squamous cell carcinomas.

Cancer letters

Tian XP,Qian D,He LR,Huang H,Mai SJ,Li CP,Huang XX,Cai MY,Liao YJ,Kung HF,Zeng YX,Xie D

Fri Oct 10 00:00:00 EDT 2014

PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.

Molecular cancer

Liu JY,Qian D,He LR,Li YH,Liao YJ,Mai SJ,Tian XP,Liu YH,Zhang JX,Kung HF,Zeng YX,Zhou FJ,Xie D

Sat Nov 23 00:00:00 EST 2013

PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.

Molecular cancer

Liu JY,Qian D,He LR,Li YH,Liao YJ,Mai SJ,Tian XP,Liu YH,Zhang JX,Kung HF,Zeng YX,Zhou FJ,Xie D

Sat Nov 23 00:00:00 EST 2013

Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.

Oncogene

Zhang B,Qian D,Ma HH,Jin R,Yang PX,Cai MY,Liu YH,Liao YJ,Deng HX,Mai SJ,Zhang H,Zeng YX,Lin MC,Kung HF,Xie D,Huang JJ

Thu Jan 05 00:00:00 EST 2012

Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.

Cancer science

Cai MY,Zhang B,He WP,Yang GF,Rao HL,Rao ZY,Wu QL,Guan XY,Kung HF,Zeng YX,Xie D

Tue Jun 01 00:00:00 EDT 2010

Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.

Cancer science

Cai MY,Zhang B,He WP,Yang GF,Rao HL,Rao ZY,Wu QL,Guan XY,Kung HF,Zeng YX,Xie D

Tue Jun 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 67-11-3 protein; FLJ20565; hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTS1; MGC8850; PIN2 interacting protein 1; pin2-interact; PIN2-interacting protein 1; PIN2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PIN2/TRF1-interacting protein; pinx1 {ECO:0000312|EMBL:ABO28828.1}; Protein 67-11-3; TRF1-interacting protein 1

Gene Aliases: 2210403I16Rik; 2610028A01Rik; 67-11-3; AU024023; LPTL; LPTS; LPTS1; PINX1; RGD1566025

UniProt ID: (Human) Q96BK5, (Rat) A4L691, (Mouse) Q9CZX5

Entrez Gene ID: (Human) 54984, (Rat) 305963, (Mouse) 72400

Molecular Function: HMG box transcription factor RNA metabolism protein nucleic acid metabolism protein