Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (81%), Dog (95%), Pig (96%), Chicken (87%), Rhesus Monkey (97%), Chimpanzee (98%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type I beta; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type I alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI4P5KIbeta; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha
Gene Aliases: PI4P5K-I[a]; PIP5K1-alpha; PIP5K1A; Pip5k1b; PIP5KIalpha; Pipk5a