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Invitrogen
PIP5K1A Polyclonal Antibody
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Product Details
PA5-29405
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PIP5K1A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.41 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (81%), Dog (95%), Pig (96%), Chicken (87%), Rhesus Monkey (97%), Chimpanzee (98%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'disheveled', the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type I beta; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type I alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI4P5KIbeta; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha
Gene Aliases: PI4P5K-I[a]; PIP5K1-alpha; PIP5K1A; PIP5KIalpha; Pipk5a
UniProt ID: (Human) Q99755, (Mouse) P70182
Entrez Gene ID: (Human) 8394, (Mouse) 18720
1-phosphatidylinositol-3-phosphate 5-kinase activity
protein binding
ATP binding
1-phosphatidylinositol-4-phosphate 5-kinase activity
kinase binding
1-phosphatidylinositol-5-kinase activity
1-phosphatidylinositol-3-phosphate 4-kinase activity
phosphatidylinositol-3,4-bisphosphate 5-kinase activity
nucleotide binding
kinase activity
phosphatidylinositol phosphate kinase activity
transferase activity
kinase
glycerophospholipid metabolic process
phosphatidylinositol biosynthetic process
phagocytosis
signal transduction
phospholipid biosynthetic process
fibroblast migration
regulation of phosphatidylinositol 3-kinase signaling
cell migration
keratinocyte differentiation
actin cytoskeleton reorganization
phosphatidylinositol phosphorylation
focal adhesion assembly
cell chemotaxis
protein targeting to plasma membrane
activation of GTPase activity
ruffle assembly
phosphorylation
phosphatidylinositol metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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