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PIST Polyclonal Antibody
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FIGURE: 1 / 3
PIST Antibody (600-401-DP1) in ICC/IF
Immunofluorescence Microscopy of PIST antibody. Cell Type: Rat colon cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PIST antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PIST is located in the cell junction, cell membrane, cell projection, cytoplasm, golgi apparatus, membrane, postsynaptic cell membrane, and synapse. Staining: PIST as red fluorescent signal.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-DP1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-PIST antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the internal region of human PIST.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CFTR-associated ligand; dJ94G16.2 PIST; FIG; PDZ domain-containing Golgi protein; fused in glioblastoma; golgi-associated PDZ and coiled-coil motif containing protein; PDZ domain-containing protein; PDZ domain-containing protein PIST; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; unnamed protein product
Gene Aliases: 2210402P09Rik; CAL; dJ94G16.2; FIG; GOPC1; PIST
UniProt ID: (Human) Q9HD26, (Mouse) Q8BH60
Entrez Gene ID: (Human) 57120, (Mouse) 94221
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