Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Osenses
PIST Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 12
PIST Antibody (OSP00107W-150UL) in IHC (P)
IHC-P on paraffin sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candel... View More
Product Details
OSP00107W-150UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from rat PIST conjugated to blue carrier protein has been used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: PIST.
Target Information
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CFTR-associated ligand; dJ94G16.2 PIST; Fused in glioblastoma; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated PDZ and coiled-coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; PDZ domain-containing protein PIST; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; PIST
Gene Aliases: 2210402P09Rik; AI844555; CAL; dJ94G16.2; FIG; GOPC; GOPC1; PIST
UniProt ID: (Human) Q9HD26, (Mouse) Q8BH60
Entrez Gene ID: (Human) 57120, (Mouse) 94221, (Rat) 309774
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support